Buell R H, Tremblay G
Cancer. 1983 May 1;51(9):1625-30. doi: 10.1002/1097-0142(19830501)51:9<1625::aid-cncr2820510913>3.0.co;2-h.
Biochemical assays of human mammary carcinomas for estrogen receptors (ER) are of proven clinical usefulness. The reliability of histochemical or immunocytochemical methods for ER localization are less well established, and less is known about the distribution of estrogen binding proteins in breast cancer. In this report we present results of a study of the uptake and retention of 3H-estradiol after in vitro incubation as visualized by thaw-mount autoradiography in five cases of biochemically estrogen receptor-positive breast cancer. These neoplasms appeared to be composed of a heterogeneous population of labeled and unlabeled tumor cells. The number of nuclear grains varied among putative target cells, and the relative percentage of labeled cells differed from area to area. Non-neoplastic mammary ductal epithelium on occasion revealed significant nuclear labeling, but stromal cells, inflammatory cells, and endothelial cells were generally negative. The possible significance of these findings is discussed.
对人乳腺癌进行雌激素受体（ER）的生化检测已被证明具有临床实用性。用于ER定位的组织化学或免疫细胞化学方法的可靠性尚未得到充分证实，关于雌激素结合蛋白在乳腺癌中的分布也知之甚少。在本报告中，我们展示了一项研究的结果，该研究通过解冻装片放射自显影术观察了5例生化雌激素受体阳性乳腺癌在体外孵育后3H-雌二醇的摄取和保留情况。这些肿瘤似乎由标记和未标记的肿瘤细胞异质性群体组成。假定的靶细胞中核颗粒的数量各不相同，标记细胞的相对百分比也因区域而异。非肿瘤性乳腺导管上皮偶尔会显示出明显的核标记，但基质细胞、炎症细胞和内皮细胞通常为阴性。本文讨论了这些发现的可能意义。
