Mackillop W J, Bizarri J P, Ward G K
Cancer Res. 1985 Sep;45(9):4360-5.
Cell suspensions derived from 2 specimens of normal human urothelium and 13 human transitional cell carcinomas were studied by discontinuous density gradient centrifugation. The histochemical and proliferative properties of the fractions were evaluated. In normal urothelium, DNA synthesis was restricted to a subpopulation of small round cells which could be partly separated from the more numerous pyramidal and giant cells on the basis of their higher physical density. Normal urothelium did not form colonies in agar. In well-differentiated tumors, the majority of cells present were low-density, elongated cells which histochemically resembled the differentiated pyramidal cells of normal tissue, but DNA synthesis and the ability to form colonies in agar were restricted to a subpopulation of high-density small round cells. Colony size analysis showed that intermediate-density bands contain cells capable of a few divisions which do not form large colonies. With increasing tumor grade, there was an apparent shift into the high-density clonogenic compartment.
通过不连续密度梯度离心法研究了来自2份正常人尿路上皮标本和13例人移行细胞癌的细胞悬液。对各组分的组织化学和增殖特性进行了评估。在正常尿路上皮中，DNA合成仅限于一小群小圆形细胞，这些细胞基于较高的物理密度可部分与数量较多的锥形细胞和巨细胞分离。正常尿路上皮在琼脂中不形成集落。在高分化肿瘤中，存在的大多数细胞是低密度、细长形细胞，其组织化学特征类似于正常组织中分化的锥形细胞，但DNA合成和在琼脂中形成集落的能力仅限于一小群高密度小圆形细胞。集落大小分析表明，中等密度条带包含能够进行几次分裂但不形成大集落的细胞。随着肿瘤分级增加，明显向高密度克隆形成区室转变。
