Ward G K, Stewart S S, Price G B, Mackillop W J
McGill Cancer Centre, McGill University, Montreal, Quebec, Canada.
Histochem J. 1987 Jun-Jul;19(6-7):337-44. doi: 10.1007/BF01680450.
A panel of 10 FITC-labelled lectins (MPA, PNA, ConA, DBA, SBA, RCA-120, WGA, UEA, GS-I, GS-II+) was applied to cryosections of seven specimens of normal urothelium. Seven of the lectins (MPA, ConA, RCA, WGA, UEA, GS-I and GS-II) showed a pattern of increasing fluorescence intensity from basal to superficial cells of the urothelium whereas PNA, DBA and SBA showed more uniform binding throughout the urothelium. Urothelial cell suspensions labelled with FITC-lectins were studied by flow cytometry to quantify the variation in binding to different cells types. Three cellular subpopulations were identified in normal urothelium on the basis of their optical properties. Fluorescence intensity due to specific lectin binding was then measured separately for each subpopulation. Although there was some variation among individual cases, a general pattern emerged in this small series. WGA, RCA, and GS-II bind in large quantities to all urothelial cells while PNA, SBA, ConA and DBA show little binding. MPA, RCA, UEA and GS-I showed the most marked increase in fluorescence intensity from basal to superficial cells as observed microscopically and quantified by flow cytometry.
将一组10种异硫氰酸荧光素（FITC）标记的凝集素（MPA、PNA、ConA、DBA、SBA、RCA - 120、WGA、UEA、GS - I、GS - II +）应用于7个正常尿路上皮标本的冰冻切片。其中7种凝集素（MPA、ConA、RCA、WGA、UEA、GS - I和GS - II）显示出从尿路上皮基底细胞到表层细胞荧光强度逐渐增加的模式，而PNA、DBA和SBA在整个尿路上皮中的结合更为均匀。通过流式细胞术研究用FITC - 凝集素标记的尿路上皮细胞悬液，以量化与不同细胞类型结合的变化。根据光学特性在正常尿路上皮中鉴定出三个细胞亚群。然后分别测量每个亚群因特异性凝集素结合而产生的荧光强度。尽管个别病例之间存在一些差异，但在这个小系列中出现了一种普遍模式。WGA、RCA和GS - II大量结合所有尿路上皮细胞，而PNA、SBA、ConA和DBA显示出很少的结合。如显微镜观察和流式细胞术量化所示，MPA、RCA、UEA和GS - I从基底细胞到表层细胞的荧光强度增加最为明显。
