Li Cencen, Cheng Xiaofang, Li Tianyu, Xie Xiujie, Li Quanxi, Chen Feng, Xu Haixia, Zhang Pengpeng, Xu Yongjie
Department of Biotechnology, college of Life Sciences, Xinyang normal University, Xinyang, China.
Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China.
Sci Data. 2025 Apr 25;12(1):700. doi: 10.1038/s41597-025-05041-5.
Skeletal muscle satellite cells are integral to muscle development and regeneration. Long non-coding RNAs (lncRNAs), a significant class of non-coding RNAs, participate in numerous biological processes and play critical roles in myogenic differentiation. However, research on the regulation of porcine satellite cell (PSC) proliferation and differentiation remains limited. To uncover key lncRNAs involved in PSC regulation, we performed strand-specific RNA-seq to comprehensively profile lncRNA and mRNA expression in PSCs at different stages of proliferation and differentiation (P24h, P48h, D18h, D28h). On average, 61.62 million clean reads were obtained across all samples. Through stringent filtering, we identified 1950 novel lncRNAs, 9367 annotated lncRNAs and 57252 mRNA transcripts for further analysis. This dataset provides valuable insight into the role of lncRNAs in PSC differentiation and contributes to a more detailed understanding of the molecular mechanisms underlying porcine muscle development.
骨骼肌卫星细胞对于肌肉发育和再生至关重要。长链非编码RNA（lncRNA）是一类重要的非编码RNA，参与众多生物过程，并在肌源性分化中发挥关键作用。然而，关于猪卫星细胞（PSC）增殖和分化调控的研究仍然有限。为了揭示参与PSC调控的关键lncRNA，我们进行了链特异性RNA测序，以全面分析PSC在增殖和分化不同阶段（P24h、P48h、D18h、D28h）的lncRNA和mRNA表达情况。所有样本平均获得6162万个clean reads。通过严格筛选，我们鉴定出1950个新的lncRNA、9367个注释lncRNA和57252个mRNA转录本用于进一步分析。该数据集为lncRNA在PSC分化中的作用提供了有价值的见解，并有助于更详细地了解猪肌肉发育的分子机制。
