Samlowski W E, Braaten B A, Daynes R A
Cell Immunol. 1985 Oct 1;95(1):1-14. doi: 10.1016/0008-8749(85)90290-4.
We have previously identified an in vivo interaction between circulating PNAhi lymphoid cells and the hepatic asialoglycoprotein receptor, which results in a protracted liver sequestration of these cells. An in vitro frozen section binding assay was developed to study the interaction of PNAhi cells with the receptor in more detail. This assay confirmed that the sequestration of PNAhi lymphoid cells by the liver was mediated by the asialoglycoprotein receptor, as binding was inhibitable by coincubation with galactose, asialoglycoproteins, chelation of divalent cations, or a specific anti-asialoglycoprotein receptor antiserum. This frozen section binding assay was utilized to demonstrate the existence of a bone marrow asialoglycoprotein receptor which was found to be capable of binding to PNAhi lymphocytes or asialoglycoproteins bound to synthetic substrates. We further established that the bone marrow receptor differed both functionally and antigenically from its hepatic analogue.
我们之前已确定循环中的高结合凝集素（PNAhi）淋巴细胞与肝脏去唾液酸糖蛋白受体之间存在体内相互作用，这导致这些细胞在肝脏中长时间滞留。为了更详细地研究PNAhi细胞与该受体的相互作用，我们开发了一种体外冰冻切片结合试验。该试验证实，肝脏对PNAhi淋巴细胞的滞留是由去唾液酸糖蛋白受体介导的，因为与半乳糖、去唾液酸糖蛋白共同孵育、二价阳离子螯合或特异性抗去唾液酸糖蛋白受体抗血清可抑制结合。利用这种冰冻切片结合试验证明了骨髓去唾液酸糖蛋白受体的存在，发现它能够结合PNAhi淋巴细胞或与合成底物结合的去唾液酸糖蛋白。我们进一步确定，骨髓受体在功能和抗原性上均与其肝脏类似物不同。
