Treichel U, Roos P H, Kolb H
Diabetes Research Institute, Düsseldorf/Federal Republic of Germany.
Eur J Cell Biol. 1989 Feb;48(1):116-20.
The hepatic asialoglycoprotein receptor (ASGP-R) was isolated from various rat tissues or freshly prepared single cell suspensions and tested for the binding to endogenous tissues or specific cell types by indirect immunofluorescence. Inhibition with N-acetyl-D-galactosamine demonstrated specificity of binding. ASGP-R binds to mesodermal tissues and to selected cells of the majority of glandular tissues but not to lining epithelia. ASGP-R stains heart muscle but not skeletal muscle. In addition, ASGP-R stains spleen cells (52%), bone marrow cells (55%), thymocytes (62%), and a fraction of peripheral blood lymphocytes (29%), which was identified as B-lymphocytes. Five different rat tumors also showed binding of ASGP-R. The binding pattern and staining intensity of peanut agglutinin and soybean agglutinin were strikingly different although the binding specificity of these lectins is related to the ASPG-R. It is concluded that considerable numbers of endogenous binding sites for the hepatic ASGP-R exist in normal tissue, even on cells which pass the liver on circulation.
从各种大鼠组织或新鲜制备的单细胞悬液中分离出肝去唾液酸糖蛋白受体（ASGP-R），并通过间接免疫荧光检测其与内源性组织或特定细胞类型的结合。用N-乙酰-D-半乳糖胺抑制证明了结合的特异性。ASGP-R与中胚层组织以及大多数腺组织的特定细胞结合，但不与衬里上皮结合。ASGP-R可染色心肌，但不能染色骨骼肌。此外，ASGP-R可染色脾细胞（52%）、骨髓细胞（55%）、胸腺细胞（62%）以及一部分外周血淋巴细胞（29%），后者被鉴定为B淋巴细胞。五种不同的大鼠肿瘤也显示出ASGP-R的结合。尽管这些凝集素的结合特异性与ASPG-R相关，但花生凝集素和大豆凝集素的结合模式和染色强度明显不同。得出的结论是，正常组织中存在大量肝ASGP-R的内源性结合位点，即使在循环中经过肝脏的细胞上也是如此。
