Ndandala Charles Brighton, Guo Yuwen, Ju Zhimin, Fachri Muhammad, Mwemi Happiness Moses, Chen Huapu
Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China.
Animals (Basel). 2025 Apr 15;15(8):1134. doi: 10.3390/ani15081134.
Insulin-like growth factors (IGFs) play crucial roles in the regulation of animal growth and reproduction. However, the functional and regulatory mechanisms underlying ovarian growth and oocyte maturation in teleosts remain unclear. In this study, the expression profiles of lncRNAs and mRNAs were analyzed in the ovaries of golden pompano () treated with IGF1 and IGF2 proteins to gain insights into the role of these two IGF ligands in the regulation of ovarian development and maturation. A total of 1494 lncRNAs and 8728 mRNAs were differentially expressed following IGF1 treatment compared with the control group. A total of 101 lncRNAs and 377 mRNAs were differentially expressed after IGF2 treatment compared to those in the control group. The results revealed that KEGG pathways enriched by target genes of the DE lncRNAs overlapped significantly with those enriched by the DE mRNAs in both the IGF1 and IGF2 groups. The key overlapping enriched pathways included ECM receptor interaction, gap junction, Hedgehog signaling pathway, Ras signaling pathway, Rap1 signaling pathway, TGF beta signaling pathway, Wnt signaling pathway, GnRH signaling pathway, progesterone-mediated oocyte maturation, oocyte meiosis, cell cycle, and MAPK signaling pathway. The differentially expressed genes (DEGs) involved in ovarian development and oocyte maturation were , , , , , , , , , , , , and , following IGF1 and IGF2 treatment. Furthermore, four lncRNAs (MSTRG.66521.1, MSTRG.49969.1, MSTRG.59923.1, and MSTRG.13767.1) for IGF1 and two (MSTRG.20896.2 and MSTRG.58123.2) for IGF2 within the lncRNA-mRNA network were found to target DEGs related to ovarian development and maturation. This suggests that IGFs may affect reproductive processes by regulating the expression of lncRNAs and mRNAs. RT-qPCR analysis revealed that these six lncRNAs showed high expression levels in the brain, pituitary, liver, and gonad tissues, indicating their potential involvement in regulating ovarian growth and development. This study elucidates the lncRNA-mRNA regulatory mechanism in response to IGF1 and IGF2 treatment during stage III of ovarian development in golden pompano, thereby deepening our understanding of its functional role.
胰岛素样生长因子（IGFs）在动物生长和繁殖的调节中发挥着关键作用。然而，硬骨鱼类卵巢生长和卵母细胞成熟的功能及调控机制仍不清楚。在本研究中，分析了经胰岛素样生长因子1（IGF1）和胰岛素样生长因子2（IGF2）蛋白处理的黄斑篮子鱼（）卵巢中长链非编码RNA（lncRNAs）和信使核糖核酸（mRNAs）的表达谱，以深入了解这两种IGF配体在卵巢发育和成熟调控中的作用。与对照组相比，IGF1处理后共有1494个lncRNAs和8728个mRNAs差异表达。与对照组相比，IGF2处理后共有101个lncRNAs和377个mRNAs差异表达。结果显示，在IGF1组和IGF2组中，差异表达lncRNAs的靶基因富集的京都基因与基因组百科全书（KEGG）通路与差异表达mRNAs富集的KEGG通路有显著重叠。关键的重叠富集通路包括细胞外基质受体相互作用、缝隙连接、刺猬信号通路、Ras信号通路、Rap1信号通路、转化生长因子β信号通路、Wnt信号通路、促性腺激素释放激素信号通路、孕酮介导的卵母细胞成熟、卵母细胞减数分裂、细胞周期和丝裂原活化蛋白激酶（MAPK）信号通路。在IGF1和IGF2处理后，参与卵巢发育和卵母细胞成熟的差异表达基因（DEGs）有 、 、 、 、 、 、 、 、 、 、 、 和 。此外，在lncRNA-mRNA网络中发现，IGF1的4个lncRNAs（MSTRG.66521.1、MSTRG.49969.1、MSTRG.59923.1和MSTRG.13767.1）和IGF2的2个lncRNAs（MSTRG.20896.2和MSTRG.58123.2）靶向与卵巢发育和成熟相关的DEGs。这表明IGFs可能通过调节lncRNAs和mRNAs的表达来影响生殖过程。逆转录-定量聚合酶链反应（RT-qPCR）分析显示，这6个lncRNAs在脑、垂体、肝脏和性腺组织中表达水平较高，表明它们可能参与调节卵巢生长和发育。本研究阐明了黄斑篮子鱼卵巢发育III期对IGF1和IGF2处理的lncRNA-mRNA调控机制，从而加深了我们对其功能作用的理解。
