Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Fisheries College, Guangdong Ocean University, Zhanjiang, China.
Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang, China.
Front Endocrinol (Lausanne). 2022 Nov 25;13:998207. doi: 10.3389/fendo.2022.998207. eCollection 2022.
Insulin-like growth factor 3 (IGF3) induces ovarian maturation in teleosts; however, research on its molecular regulatory mechanism remains deficient. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in various biological processes, including reproduction. In this study, circRNAs and miRNAs involved in IGF3-induced ovarian maturation were evaluated in spotted scat (). In ovarian tissues, we identified 176 differentially expressed (DE) circRNAs and 52 DE miRNAs between IGF3 treatment and control groups. Gene Ontology (GO) enrichment analyses showed that host genes of DE circRNAs and target genes of DE miRNAs were enriched for various processes with a high degree of overlap, including cellular process, reproduction, reproductive process, biological adhesion, growth, extracellular region, cell junction, catalytic activity, and transcription factor activity. Enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included cell adhesion molecules, ECM-receptor interaction, regulation of actin cytoskeleton, focal adhesion, cell cycle, Hedgehog signaling pathway, phosphatidylinositol signaling system, PI3K-Akt signaling pathway, Apelin signaling pathway, Notch signaling pathway, insulin signaling pathway, and Rap1 signaling pathway. A circRNA-miRNA-mRNA regulatory network was constructed, including DE genes involved in reproduction (e.g., oocyte maturation, oocyte meiosis, and ECM remodeling), such as , , , , , and . According to the regulatory network and tissue distribution, we identified one circRNA (Lachesis_group5:6245955|6270787) and three miRNAs (novel_miR_622, novel_miR_980, and novel_miR_64) that may exert regulatory effects in IGF3-induced ovarian maturation in . Taken together, this study provides a novel insight into the molecular mechanisms by which IGF3 functions in ovaries and highlights the effects of circRNAs and miRNAs in reproduction in .
胰岛素样生长因子 3（IGF3）诱导硬骨鱼类的卵巢成熟；然而，其分子调控机制的研究仍然不足。环状 RNA（circRNA）和 microRNA（miRNA）参与多种生物学过程，包括生殖。本研究评估了 IGF3 诱导斑点叉尾鮰卵巢成熟过程中涉及的 circRNA 和 miRNA。在卵巢组织中，我们在 IGF3 处理组和对照组之间鉴定出 176 个差异表达（DE）circRNA 和 52 个 DE miRNA。基因本体论（GO）富集分析表明，DE circRNA 的宿主基因和 DE miRNA 的靶基因富集了各种高度重叠的过程，包括细胞过程、生殖、生殖过程、生物粘附、生长、细胞外区、细胞连接、催化活性和转录因子活性。富含京都基因与基因组百科全书（KEGG）通路包括细胞粘附分子、细胞外基质受体相互作用、肌动蛋白细胞骨架调节、焦点粘连、细胞周期、Hedgehog 信号通路、磷酸肌醇信号系统、PI3K-Akt 信号通路、Apelin 信号通路、Notch 信号通路、胰岛素信号通路和 Rap1 信号通路。构建了一个 circRNA-miRNA-mRNA 调控网络，包括参与生殖的 DE 基因（如卵母细胞成熟、卵母细胞减数分裂和细胞外基质重塑），如、、、、、和。根据调控网络和组织分布，我们鉴定出一个 circRNA（Lachesis_group5:6245955|6270787）和三个 miRNA（novel_miR_622、novel_miR_980 和 novel_miR_64）可能在 IGF3 诱导斑点叉尾鮰卵巢成熟中发挥调节作用。总之，本研究为 IGF3 在卵巢中的作用提供了一个新的分子机制视角，并强调了 circRNA 和 miRNA 在生殖中的作用。
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