El-Saka Eman M, El-Wishy Abou Bakr A, Moawad Adel R, Ibrahim Sally, Ibrahim Saber, Shahat Abdallah M
Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Division of Animal Science, College of Agriculture, Family Sciences, and Technology, Fort Valley State University, Fort Valley, GA 31030, USA.
Animals (Basel). 2025 Apr 16;15(8):1147. doi: 10.3390/ani15081147.
Oocytes are exposed to various stressors during in vitro maturation (IVM). Antioxidant supplementation during IVM can mitigate oxidative stress. We investigated the effects of supplementing IVM medium with novel flavonoid-enriched antioxidant nanoformulations, namely, EMD-300 and EMP3-H200, on oocyte IVM and analyzed the expression of oxidative stress, apoptosis, and pluripotency genes in buffalo. Cumulus oocyte complexes (COCs) obtained from buffalo ovaries were matured in IVM medium supplemented with either EMD-300 or EMP3-H200 at 0.5% and 1.0% for 22 h. Following IVM, nuclear maturation, gene expression, and the levels of total antioxidant capacity (TAC) and malondialdehyde (MDA) were analyzed. Nuclear maturation was lower ( < 0.001) for the 1.0% EMD-300 group than other groups. The expressions of the GPX4, SOD, CAT, and ATF6 genes were lower ( < 0.05) in the 0.5% EMD-300 and EMP3-H200 groups than in the control. OCT4 gene expression was higher ( < 0.05) for the treated groups than control group. The level of TAC in spent IVM medium was higher for the 0.5% EMD-300 and EMP3-H200 groups than for the control. However, the MDA concentrations were lower. In conclusion, supplementing IVM medium with EMD-300 or EMP3-H200 at 0.5% improved nuclear maturation of buffalo oocytes better than 1.0%. Our findings suggest that these compounds had antioxidant effects, which assures their ability in protecting oocytes against oxidative stress.
卵母细胞在体外成熟（IVM）过程中会受到各种应激源的影响。IVM期间补充抗氧化剂可以减轻氧化应激。我们研究了在IVM培养基中添加新型富含黄酮类抗氧化剂纳米制剂（即EMD - 300和EMP3 - H200）对水牛卵母细胞IVM的影响，并分析了氧化应激、凋亡和多能性基因在水牛中的表达。从水牛卵巢获得的卵丘卵母细胞复合体（COCs）在添加0.5%和1.0%的EMD - 300或EMP3 - H200的IVM培养基中成熟22小时。IVM后，分析核成熟、基因表达以及总抗氧化能力（TAC）和丙二醛（MDA）水平。1.0% EMD - 300组的核成熟率低于其他组（<0.001）。0.5% EMD - 300和EMP3 - H200组中GPX4、SOD、CAT和ATF6基因的表达低于对照组（<0.05）。处理组的OCT4基因表达高于对照组（<0.05）。0.5% EMD - 300和EMP3 - H200组用过的IVM培养基中的TAC水平高于对照组。然而，MDA浓度较低。总之，在IVM培养基中添加0.5%的EMD - 300或EMP3 - H200比添加1.0%能更好地改善水牛卵母细胞的核成熟。我们的研究结果表明，这些化合物具有抗氧化作用，这确保了它们保护卵母细胞免受氧化应激的能力。
