Ritika Ritika, Saini Sudha, Shavi Shavi, Ramesh P N, Selokar Naresh L, Ludri Ashutosh, Singh Manoj Kumar
Animal Biotechnology Division, ICAR-National Dairy Research Institute, Karnal, Haryana, India.
Animal Physiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana, India.
Vet World. 2024 Nov;17(11):2433-2442. doi: 10.14202/vetworld.2024.2433-2442. Epub 2024 Nov 5.
Buffalo is the principal dairy animal and plays a major role in the economic growth of the dairy industry, contributing nearly 50% of the country's milk production. The Buffalo core body temperature is typically 38.5°C, but it can rise to 41.5°C in the summer, causing heat stress, which leads to the generation of reactive oxygen species or oxidative stress and affects the reproductive physiology of animals. Curcumin acts as an antioxidant, improves cellular development, and combats the effect of heat stress on produced embryos. This study aimed to examine the impact of curcumin on developmental competence and the expression of important genes under normal and heat-stressed conditions during embryo production in buffalo.
Group-1: All embryo production steps (i.e., maturation [IVM], fertilization [IVF], and culture [IVC]) were conducted at 38.5°C. The presumed zygotes were cultured in media supplemented with different concentrations of curcumin, that is, 0 μM, 5 μM, and 10 μM of curcumin. Group-2: All embryo production steps (i.e., IVM, IVF, and IVC) were carried out at 38.5°C. The presumed zygotes were cultured in media supplemented with different concentrations of curcumin, that is, 0 μM, 5 μM, and 10 μM of curcumin, but the early cleaved embryos were exposed to heat stress (39.5°C) for 2 h after 48 h of IVF and then cultured at 38.5°C for embryo production.
Blastocyst production was 16.63 ± 1.49%, 21.46 ± 0.67%, and 6.50 ± 1.17% at control, 5 μM and 10 μM of curcumin at 38.5°C, respectively, whereas at 39.5°C, it was 8.59 ± 1.20%, 15.21 ± 1.31%, and 3.03 ± 1.20% at control, 5 μM and 10 μM curcumin, respectively. The blastocyst rate was found to be significantly higher (p < 0.05) at 5 μM curcumin compared with the control or 10 μM at 38.5°C and 39.5°C. The antioxidant, antiapoptotic, and pluripotency-related genes exhibited higher (p < 0.05) expression in the presence of 5 μM curcumin compared to 10 μM or control at both temperatures.
Curcumin supplementation in embryo culture media effectively enhances embryo production and mitigates the adverse effects of heat stress.
水牛是主要的乳用动物，在乳业经济增长中发挥着重要作用，贡献了该国近50%的牛奶产量。水牛的核心体温通常为38.5°C，但在夏季可升至41.5°C，导致热应激，进而产生活性氧或氧化应激，影响动物的生殖生理。姜黄素作为一种抗氧化剂，可促进细胞发育，并对抗热应激对所产生胚胎的影响。本研究旨在考察姜黄素在正常和热应激条件下对水牛胚胎生产过程中发育能力及重要基因表达的影响。
第1组：所有胚胎生产步骤（即体外成熟[IVM]、体外受精[IVF]和体外培养[IVC]）均在38.5°C下进行。将推定的受精卵在添加不同浓度姜黄素（即0 μM、5 μM和10 μM姜黄素）的培养基中培养。第2组：所有胚胎生产步骤（即IVM、IVF和IVC）均在38.5°C下进行。将推定的受精卵在添加不同浓度姜黄素（即0 μM、5 μM和10 μM姜黄素）的培养基中培养，但早期分裂胚胎在IVF 48小时后暴露于热应激（39.5°C）2小时，然后在38.5°C下培养以进行胚胎生产。
在38.5°C时，对照组、5 μM和10 μM姜黄素组的囊胚率分别为16.63±1.49%、21.46±0.67%和6.50±1.17%；而在39.5°C时，对照组、5 μM和10 μM姜黄素组的囊胚率分别为8.59±1.20%、15.21±1.31%和3.03±1.20%。发现在38.5°C和39.5°C时，5 μM姜黄素组的囊胚率显著高于对照组或10 μM姜黄素组（p<0.05）。在两个温度下，与10 μM或对照组相比，5 μM姜黄素存在时抗氧化、抗凋亡和多能性相关基因的表达更高（p<0.05）。
在胚胎培养基中添加姜黄素可有效提高胚胎产量，并减轻热应激的不利影响。
