Peng Junjie, Zhang Linhan, Li Jiangling, Lv Xuebin, Liu Rui, Chen Jianlin, Wang Gang, Gao Rong
College of Life Science, Sichuan University, Chengdu 610065, China.
National Engineering Research Center for Biomaterial, Sichuan University, Chengdu 610065, China.
Biology (Basel). 2025 Apr 2;14(4):366. doi: 10.3390/biology14040366.
China's livestock industry grapples with challenges posed by infectious diseases and the misuse of antibiotics, resulting in a heightened risk of drug-resistant pathogens. This study explored the immunomodulatory effects of co-expressing porcine interleukin 3, 7, and 15 in , denoted as Po1h-IL-3/7/15. A 42-day experiment involving mouse immunization and pathogen challenge was conducted, during which in vivo assessments of antibodies, immune-related cells, and gene expression were detected following oral administration of Po1h-IL-3/7/15. Immunological alterations in mice were analyzed using flow cytometry, qRT-PCR, ELISA, and HE staining. Notably, the serum IgG and intestinal sIgA levels in the Po1h-IL-3/7/15 group were substantially elevated compared to the control groups ( < 0.01), so were the contents of IL-7, IL-15, IFN-γ, IL-22, IL-23, and TNF-α. Furthermore, there was a marked increase in naïve T cells and central memory T cells, accompanied by a significant decrease in regulatory T cells in peripheral blood. Post-challenge with or , the expression levels of BD2, IL-1β, IL-8, Jak1, RegⅢ, S100A8, STAT1, and TNF-α genes in the intestines of the Po1h-IL-3/7/15 group were markedly higher than those in the control groups ( < 0.01). Following the challenges, the survival rate of the Po1h-IL-3/7/15 group was 100%, a significant increase compared to the 20% and 40% survival rates observed in the control groups ( < 0.05). These results confirm that IL-3/7/15 significantly boosts innate immunity, humoral and cell-mediated immune responses, and intestinal mucosal immunity in mice, enhancing resistance to bacterial infections and exhibiting potent protective effects.
中国畜牧业面临着传染病和抗生素滥用带来的挑战，导致耐药病原体风险增加。本研究探讨了共表达猪白细胞介素3、7和15（命名为Po1h-IL-3/7/15）的免疫调节作用。进行了一项为期42天的实验，涉及小鼠免疫和病原体攻击，在此期间，口服Po1h-IL-3/7/15后检测抗体、免疫相关细胞和基因表达的体内评估。使用流式细胞术、qRT-PCR、ELISA和HE染色分析小鼠的免疫变化。值得注意的是，与对照组相比，Po1h-IL-3/7/15组的血清IgG和肠道sIgA水平显著升高（<0.01），IL-7、IL-15、IFN-γ、IL-22、IL-23和TNF-α的含量也显著升高。此外，外周血中幼稚T细胞和中枢记忆T细胞显著增加，调节性T细胞显著减少。用或攻击后，Po1h-IL-3/7/15组肠道中BD2、IL-1β、IL-8、Jak1、RegⅢ、S100A8、STAT1和TNF-α基因的表达水平显著高于对照组（<0.01）。攻击后，Po1h-IL-3/7/15组的存活率为100%，与对照组观察到的20%和40%的存活率相比显著提高（<0.05）。这些结果证实，IL-3/7/15显著增强小鼠的先天免疫、体液和细胞介导的免疫反应以及肠道黏膜免疫，增强对细菌感染的抵抗力并表现出强大的保护作用。
