MicroRNAs (miRNAs) play crucial roles in regulating various biological processes in crustaceans, including stress responses. Under acute low salinity stress conditions, miRNAs exhibit dynamic expression patterns that significantly influence the physiological and molecular responses of the shrimp. However, research on miRNAs in is very limited, and their functions under low salinity stress remain unclear. In this study, by using high-throughput sequencing technology, we identified miRNAs and investigated their regulatory mechanism in under low salinity stress. A total of 118 miRNAs were differentially expressed after low salinity exposure. These miRNAs were found to target genes involved in metabolism, pathogen infection, immune response and stress signaling pathways. By modulating the expression of these target genes, miRNAs were able to fine-tune the stress response of , thereby enhancing its tolerance to low salinity. This study provides new insights into the regulatory roles of miRNAs in the stress response of aquatic organisms and suggests potential targets for genetic improvement to enhance stress tolerance in aquaculture.