National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China.
Fish Shellfish Immunol. 2024 Oct;153:109827. doi: 10.1016/j.fsi.2024.109827. Epub 2024 Aug 10.
MicroRNAs (miRNAs) are a category of small non-coding RNAs regarded as vital regulatory factors in various biological processes, especially immune regulation. The differently expressed miRNAs in Macrobrachium rosenbergii after the challenge of Vibrio parahaemolyticus were identified using high-throughput sequencing. A total of 18 known as well as 12 novel miRNAs were markedly differently expressed during the bacterial infection. The results of the target gene prediction and enrichment analysis indicated that a total of 230 target genes involved in a large variety of signaling pathways and biological processes were mediated by the miRNAs identified in the current research. Additionally, the effects of novel-miR-56, a representative differentially expressed miRNA identified in the previous infection experiment, on the immune-related gene expression in M. rosenbergii were explored. The expression of the immune-related genes including Spätzle1(Spz1), Spz4, Toll-like receptor 1 (TLR1), TLR2, TLR3, immune deficiency (IMD), myeloid differentiation factor 88 (MyD88), anti-lipopolysaccharide factor 1 (ALF1), crustin1, as well as prophenoloxidase (proPO) was significantly repressed in the novel-miR-56-overexpressed prawns. The expression of these genes tested in the novel-miR-56-overexpressed M. rosenbergii was still signally lower than the control in the subsequent V. parahaemolyticus challenge, despite the gene expression in each treatment increased significantly after the infection. Additionally, the cumulative mortality of the agomiR-56-treated prawns was significantly higher than the other treatments post the bacterial challenge. These results suggested that novel-miR-56 might function as a negative regulator of the immune-related gene expression of M. rosenbergii in the innate immune defense against V. parahaemolyticus.
微小 RNA（miRNA）是一类小型非编码 RNA，被认为是各种生物过程中至关重要的调节因子，特别是在免疫调节中。通过高通量测序鉴定了罗氏沼虾在副溶血弧菌攻毒后差异表达的 microRNAs。在细菌感染过程中，共有 18 个已知和 12 个新的 microRNA 明显差异表达。靶基因预测和富集分析的结果表明，由本研究中鉴定的 microRNA 介导的 230 个总靶基因参与了多种信号通路和生物过程。此外，还探讨了先前感染实验中鉴定的代表性差异表达 microRNA 新-miR-56 对罗氏沼虾免疫相关基因表达的影响。免疫相关基因 Spätzle1（Spz1）、Spz4、Toll 样受体 1（TLR1）、TLR2、TLR3、免疫缺陷（IMD）、髓样分化因子 88（MyD88）、抗脂多糖因子 1（ALF1）、crustin1 和原酚氧化酶（proPO）的表达在新-miR-56 过表达的对虾中显著受到抑制。在新-miR-56 过表达的罗氏沼虾中检测到这些基因的表达在随后的副溶血弧菌攻毒后仍然明显低于对照，但每种处理的基因表达在感染后均显著增加。此外，agomiR-56 处理的对虾在细菌攻毒后的累积死亡率明显高于其他处理。这些结果表明，新-miR-56 可能在罗氏沼虾先天免疫防御副溶血弧菌中作为免疫相关基因表达的负调节剂发挥作用。
