Ji Jing, Wang Qiaohuang, Li Shuigen, Chen Yanting, Zhang Jiexin, Yu Hanxiu, Xu Jinzhen, Li Miaomiao, Zheng Renhao, Lin Nan, Zhang Ziping
Fujian Agriculture and Forestry University, Fuzhou, China.
Fujian Provincial Fisheries Technology Extension Center, Fuzhou, China.
Front Vet Sci. 2024 Aug 21;11:1464291. doi: 10.3389/fvets.2024.1464291. eCollection 2024.
To investigate the different mechanisms of in response to acute and chronic hypotonic stress, RNA sequencing technology was employed to profile the gene expression patterns in the gill, hepatopancreas, and hemocyte at 0, 6, 48, and 72 h post acute hypotonic stress treatment (with salinity immediately decreased from 20 psu to 4 psu) and at 0, 2, 10, 15 days during chronic hypotonic stress treatment (with salinity gradually decreased from 20 psu to 4 psu). The control group (SC) was maintained at a constant salinity of 20 psu. Differentially expressed genes (DEGs) were identified, followed by further validation using real-time quantitative reverse transcription PCR (RT-qPCR). A total of 34,217 genes were expressed through sequencing. Compared with the control group, 8,503 DEGs were identified in the acute hypotonic stress group, comprising 3,266 up-regulated and 5,237 down-regulated genes. In the chronic hypotonic stress group, 8,900 DEGs were detected, including 3,019 up-regulated and 5,881 down-regulated genes. Gene Ontology (GO) functional annotation analysis indicated that DEGs were primarily enriched in biological processes such as cellular and metabolic processes, cellular components like membrane and other cellular components, and molecular functions including structural binding and catalytic activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that DEGs were predominantly concentrated in five major pathways: metabolism, genetic information processing, environmental information processing, cellular processes, and biological systems. These pathways encompassed antigen processing and presentation, the NOD-like receptor signaling pathway, the Toll-like receptor signaling and cell apoptosis. The RT-qPCR validation of 11 DEGs (70, 90, 3, , 12, 4, 88, , 7, 9 and ) demonstrated that the trends observed in the quantitative results were consistent with those from the transcriptome analysis, thereby validating the reliability of transcriptome sequencing data. This study identified that hypotonic stress triggers physiological responses in to both acute and chronic hypotonic conditions, offering valuable insights into the expression patterns of functional genes in the gills, hepatopancreas, and hemocytes of under such stress. These findings provide foundational data and a theoretical basis for further research into the regulatory mechanism of in response to hypotonic stress.
为了研究[物种名称]对急性和慢性低渗胁迫的不同响应机制，采用RNA测序技术分析了急性低渗胁迫处理（盐度从20 psu立即降至4 psu）后0、6、48和72小时以及慢性低渗胁迫处理（盐度从20 psu逐渐降至4 psu）期间0、2、10、15天鳃、肝胰腺和血细胞中的基因表达模式。对照组（SC）维持在20 psu的恒定盐度。鉴定出差异表达基因（DEG），随后使用实时定量逆转录PCR（RT-qPCR）进行进一步验证。通过测序共表达了34,217个基因。与对照组相比，急性低渗胁迫组鉴定出8,503个DEG，包括3,266个上调基因和5,237个下调基因。在慢性低渗胁迫组中，检测到8,900个DEG；包括3,019个上调基因和5,881个下调基因。基因本体（GO）功能注释分析表明，DEG主要富集在细胞和代谢过程等生物学过程、膜和其他细胞成分等细胞成分以及结构结合和催化活性等分子功能中。京都基因与基因组百科全书（KEGG）通路富集分析表明，DEG主要集中在五个主要通路：代谢、遗传信息处理、环境信息处理、细胞过程和生物系统。这些通路包括抗原加工和呈递、NOD样受体信号通路、Toll样受体信号传导和细胞凋亡。对11个DEG（70、90、3、[此处原文缺失部分信息]、12、4、88、[此处原文缺失部分信息]、7、9和[此处原文缺失部分信息]）的RT-qPCR验证表明，定量结果中观察到的趋势与转录组分析结果一致，从而验证了转录组测序数据的可靠性。本研究确定低渗胁迫会触发[物种名称]对急性和慢性低渗条件的生理反应，为研究该物种在这种胁迫下鳃、肝胰腺和血细胞中功能基因的表达模式提供了有价值的见解；这些发现为进一步研究[物种名称]对低渗胁迫的调控机制提供了基础数据和理论依据。
