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质体基因组序列揭示了韩国特有物种(天门冬科)作为复合体一部分的情况。

Plastome Sequences Uncover the Korean Endemic Species (Asparagaceae) as Part of the . Complex.

作者信息

Jung Joonhyung, Kim Hyuk-Jin, Kim Joo-Hwan

机构信息

Division of Forest Biodiversity, Korea National Arboretum, Pocheon 11186, Republic of Korea.

Department of Life Sciences, Gachon University, Seongnam 13120, Republic of Korea.

出版信息

Genes (Basel). 2025 Mar 29;16(4):398. doi: 10.3390/genes16040398.

Abstract

Y.S.Kim, B.U.Oh & C.G.Jang (Asparagaceae Juss.), a Korean endemic species, has been described based on its erect stem, tubular perianth shape, and pedicel length. However, its taxonomic status remains unclear due to limited molecular data. This study presents the complete plastid genomes (plastomes) of two individuals and its close relative, . (Mill.) Druce var. (C.Morren & Decne.) H.Hara. The plastomes, ranging from 154,578 to 154,579 base pairs (bp), are identical to those of . A.Gray, . var. , and another Korean endemic species, . Y.S.Kim, B.U.Oh & C.G.Jang. All contain 78 plastid protein-coding genes (PCGs), 30 tRNA genes, and four rRNA genes, except for the pseudogene A. Phylogenetic analyses using 78 plastid PCGs and whole intergenic spacer (IGS) regions strongly support the three sections within Mill. and show that . and its variety are nested within . , . , and . . Given the limited genomic variation and phylogenetic relationships, we propose treating . , . , and . as part of the . complex, despite their morphological differences. This study offers valuable putative molecular markers for species identification and supports the application of plastome-based super-barcoding in the morphologically diverse genus .

摘要

Y.S.金、B.U.吴和C.G.张(天门冬科),一种韩国特有物种,是根据其直立茎、管状花被形状和花梗长度来描述的。然而,由于分子数据有限,其分类地位仍不清楚。本研究展示了两个个体及其近缘种[具体物种未提及]的完整质体基因组(质体基因组)。(米尔恩)德鲁斯变种[具体变种未提及](C.莫伦和德克内)原田。质体基因组大小在154,578至154,579碱基对(bp)之间,与[其他物种未提及]A.格雷、[其他变种未提及]变种以及另一种韩国特有物种Y.S.金、B.U.吴和C.G.张的质体基因组相同。所有这些都包含78个质体蛋白编码基因(PCG)、30个tRNA基因和4个rRNA基因,但假基因A除外。使用78个质体PCG和整个基因间隔区(IGS)区域进行的系统发育分析有力地支持了米尔恩分类下的三个组,并表明[具体物种未提及]及其变种嵌套在[其他物种未提及]、[其他物种未提及]和[其他物种未提及]之中。鉴于有限的基因组变异和系统发育关系,尽管它们在形态上存在差异,我们建议将[具体物种未提及]、[具体物种未提及]和[具体物种未提及]视为[具体属未提及]复合体的一部分。本研究为物种鉴定提供了有价值的假定分子标记,并支持在形态多样的[具体属未提及]属中应用基于质体基因组的超级条形码技术。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5988/12027285/85daa718e448/genes-16-00398-g001.jpg

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