He Peize, Cai Yanling, Wang Yanzi, Wang Zhiqing, Lyu Yaqing, Li Tao, Zhang Xingtan, Zhou Shaoqun
College of Informatics, Huazhong Agricultural University, Wuhan 430070, China.
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Genes (Basel). 2025 Apr 21;16(4):470. doi: 10.3390/genes16040470.
Functional alleles of host plant susceptibility genes (S genes) can exacerbate the severity of diseases by enhancing pathogen compatibility. Genetic editing of the targeted host S genes has demonstrated remarkable efficacy in conferring broad-spectrum resistance across multiple crop species. We have previously identified a homolog () in the host plant as a susceptibility gene towards late blight caused by .
In this study, we performed a detailed characterization of tissue-specific and -inducible expression patterns of this gene, and the subcellular localization of its encoded protein product.
Similar to phenotypes of two reported edited lines, two newly generated genetically edited lines of demonstrated enhanced resistance against without obvious growth and developmental abnormality. Phytohormonal quantifications and reactive oxygen species measurements showed that an line had lower constitutive abscisic acid contents and depleted reactive oxygen species burst induced by pathogen-associated molecular pattern. Further comparative transcriptomic analyses revealed that the expression of defense-related genes is disproportionally up-regulated in the line.
In summary, our findings confirmed as a functional host susceptibility gene towards late blight and shed light on the potential molecular mechanism underlying its function.
宿主植物感病基因（S基因）的功能性等位基因可通过增强与病原体的兼容性来加剧病害的严重程度。对靶向宿主S基因进行基因编辑已在多种作物中展现出显著的广谱抗病效果。我们之前已在宿主植物中鉴定出一个同源基因（）作为对由引起的晚疫病的感病基因。
在本研究中，我们对该基因的组织特异性和诱导型表达模式及其编码蛋白产物的亚细胞定位进行了详细表征。
与两个已报道的编辑系的表型相似，两个新产生的基因编辑系对表现出增强的抗性，且无明显的生长发育异常。植物激素定量分析和活性氧测量表明，一个系的组成型脱落酸含量较低，且病原体相关分子模式诱导的活性氧爆发减少。进一步的比较转录组分析显示，防御相关基因的表达在系中上调程度不均衡。
总之，我们的研究结果证实为对晚疫病起作用的功能性宿主感病基因，并揭示了其潜在的分子功能机制。
