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3
The Solanum demissum R8 late blight resistance gene is an Sw-5 homologue that has been deployed worldwide in late blight resistant varieties.茄属植物德米森R8晚疫病抗性基因是Sw-5的同源基因，已在全球范围内应用于晚疫病抗性品种中。
Theor Appl Genet. 2016 Sep;129(9):1785-96. doi: 10.1007/s00122-016-2740-0. Epub 2016 Jun 17.
4
Emerging fungal threats to animal, plant and ecosystem health.新兴真菌对动物、植物和生态系统健康的威胁。
Nature. 2012 Apr 11;484(7393):186-94. doi: 10.1038/nature10947.

（晚疫病）马铃薯离体叶片感染测定

(Late blight) Infection Assay in a Detached Leaf of Potato.

作者信息

Karki Hari S, Halterman Dennis A

机构信息

Department of Agriculture-Agricultural Research Service, Madison, U.S.A.

出版信息

Bio Protoc. 2021 Feb 20;11(4):e3926. doi: 10.21769/BioProtoc.3926.

DOI:10.21769/BioProtoc.3926
PMID:33732813
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7952958/
Abstract

is a hemibiotroph oomycete that primarily infects potato and tomato. It infects stems, leaves, and tubers and fruits of potato and tomato. High throughput and reproducible infection assays are prerequisites to find sources of resistance in any crop. In this protocol, we describe a detached leaf assay (DLA) for conducting the virulence assay of in potato leaves. A late blight infection assay using a potato detached leaf is a semi-high throughput assay in which hundreds of plants can be screened in a laboratory setting.

摘要

是一种半活体营养卵菌，主要感染马铃薯和番茄。它会感染马铃薯和番茄的茎、叶、块茎及果实。高通量且可重复的感染试验是在任何作物中寻找抗性来源的先决条件。在本方案中，我们描述了一种用于进行马铃薯叶片致病力测定的离体叶片试验（DLA）。使用马铃薯离体叶片进行晚疫病感染试验是一种半高通量试验，在实验室环境中可以筛选数百株植物。