（晚疫病）马铃薯离体叶片感染测定
(Late blight) Infection Assay in a Detached Leaf of Potato.
作者信息
Karki Hari S, Halterman Dennis A
机构信息
Department of Agriculture-Agricultural Research Service, Madison, U.S.A.
出版信息
Bio Protoc. 2021 Feb 20;11(4):e3926. doi: 10.21769/BioProtoc.3926.
Abstract
is a hemibiotroph oomycete that primarily infects potato and tomato. It infects stems, leaves, and tubers and fruits of potato and tomato. High throughput and reproducible infection assays are prerequisites to find sources of resistance in any crop. In this protocol, we describe a detached leaf assay (DLA) for conducting the virulence assay of in potato leaves. A late blight infection assay using a potato detached leaf is a semi-high throughput assay in which hundreds of plants can be screened in a laboratory setting.
摘要
是一种半活体营养卵菌，主要感染马铃薯和番茄。它会感染马铃薯和番茄的茎、叶、块茎及果实。高通量且可重复的感染试验是在任何作物中寻找抗性来源的先决条件。在本方案中，我们描述了一种用于进行马铃薯叶片致病力测定的离体叶片试验（DLA）。使用马铃薯离体叶片进行晚疫病感染试验是一种半高通量试验，在实验室环境中可以筛选数百株植物。
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