He Rifeng, Lian Ziling, Cheng Zhongjun, Liu Yang, Peng Xiaoyan, Wang Yong, Ma Hang, Zhou Xue, Ge Fahuan
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Bijie Institute of Traditional Chinese Medicine, Bijie 551700, China.
Foods. 2025 Apr 9;14(8):1301. doi: 10.3390/foods14081301.
Cili ( Tratt) fruit is a nutrient-rich edible plant known for its antioxidant and hepatoprotective properties. However, conventional extraction methods often lead to the degradation of its bioactive compounds. In this study, we developed a low-temperature homogenate-assisted high-pressure disruption extraction (HHPD) method to obtain a phytochemically enriched cili fruit extract (HHPD-CFE). The chemical characterization of the HHPD-CFE showed that it contained higher levels of polyphenols, polysaccharides, and superoxide dismutase (SOD) than those in conventional squeeze extraction. The hepatoprotective effects of the HHPD-CFE were evaluated in oxidative stress-induced liver injury and hepatic fibrosis models. The HHPD-CFE mitigated oxidative damage by reducing malondialdehyde while enhancing SOD and glutathione activity. Additionally, the HHPD-CFE inhibited the activation of hepatic stellate cells (HSC-T6) and reduced collagen deposition, suggesting a protective role against liver fibrosis. These findings support that the HHPD-CFE is a promising botanical extract with enriched bioactive compounds and liver-protective properties. This study supports the potential application of optimized extraction techniques to preserve thermosensitive compounds and improve the efficacy of functional foods for liver health.
刺梨（缫丝花）果实是一种营养丰富的可食用植物，以其抗氧化和保肝特性而闻名。然而，传统的提取方法往往会导致其生物活性化合物的降解。在本研究中，我们开发了一种低温匀浆辅助高压破碎提取（HHPD）方法，以获得富含植物化学物质的刺梨果实提取物（HHPD-CFE）。HHPD-CFE的化学表征表明，它所含的多酚、多糖和超氧化物歧化酶（SOD）水平高于传统压榨提取法。在氧化应激诱导的肝损伤和肝纤维化模型中评估了HHPD-CFE的保肝作用。HHPD-CFE通过降低丙二醛同时增强SOD和谷胱甘肽活性来减轻氧化损伤。此外，HHPD-CFE抑制肝星状细胞（HSC-T6）的活化并减少胶原蛋白沉积，表明其对肝纤维化具有保护作用。这些发现支持HHPD-CFE是一种具有丰富生物活性化合物和肝脏保护特性的有前景的植物提取物。本研究支持优化提取技术在保存热敏化合物和提高功能性食品对肝脏健康功效方面的潜在应用。
