Morales-Barron Bruce Manuel, Larios-Serrato Violeta, Morales-García Yolanda Elizabeth, Quintero-Hernández Verónica, Estrada-de Los Santos Paulina, Muñoz-Rojas Jesús
Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prolongación Carpio y Plan de Ayala, Col. Santo Tomas, Alcaldía Miguel Hidalgo, Ciudad de México C.P. 11340, Mexico.
Ecology and Survival of Microorganisms Group, Laboratorio de Ecología Molecular Microbiana, Benemérita Universidad Autónoma de Puebla (BUAP), Edificio IC11, Ciudad Universitaria, Colonia Jardines de San Manuel, Puebla C.P. 72570, Mexico.
Life (Basel). 2025 Mar 28;15(4):554. doi: 10.3390/life15040554.
In the present study, the strain EMM was isolated from germinated autochthonous red maize seeds, which were harvested in a region of San Diego-Buenavista, Papalotla, Tlaxcala, Mexico, where cobs with fungal infections have been observed. This fungal strain caused wilting in the maize seedlings. EMM-1 was tested for its ability to inhibit EMM, both in culture media and in association with maize plantlets. EMM-1 inhibited the growth of EMM under all culture media conditions explored. The ability of EMM-1 to inhibit the growth of EMM associated with plants was evaluated in both a hydroponic system and in vermiculite. In both systems, EMM-1 strongly inhibited the growth of EMM. The dry weight of root plants infected with EMM and inoculated with EMM-1 increased to 0.43 g, while that of plants infected only with EMM reached just 0.19 g under hydroponic conditions. However, no differences were observed under vermiculite conditions. The dry weight of the aerial region of plants infected with EMM and inoculated with EMM-1 was greater than that of plants infected only with EMM, both under hydroponic and vermiculite conditions. These results indicate that EMM-1 inhibits the infection caused by EMM, thereby improving plant growth. Moreover, the genome analysis of EMM-1 revealed the presence of several genes that potentially encode for antimicrobial compounds, which could strengthen the potential use of EMM-1 as a biocontrol agent in maize plants.
在本研究中，EMM菌株是从当地发芽的红色玉米种子中分离出来的，这些种子收获于墨西哥特拉斯卡拉州帕帕洛特拉的圣地亚哥 - 布埃纳维斯塔地区，在那里已观察到有真菌感染的玉米棒。这种真菌菌株导致玉米幼苗枯萎。对EMM - 1在培养基中以及与玉米幼苗共同培养时抑制EMM的能力进行了测试。在所有探索的培养基条件下，EMM - 1均能抑制EMM的生长。在水培系统和蛭石中评估了EMM - 1抑制与植物相关的EMM生长的能力。在这两种系统中，EMM - 1都强烈抑制了EMM的生长。在水培条件下，感染EMM并接种EMM - 1的植物根系干重增加到0.43克，而仅感染EMM的植物根系干重仅为0.19克。然而，在蛭石条件下未观察到差异。在水培和蛭石条件下，感染EMM并接种EMM - 1的植物地上部分干重均大于仅感染EMM的植物。这些结果表明，EMM - 1抑制了EMM引起的感染，从而促进了植物生长。此外，对EMM - 1的基因组分析揭示了几个可能编码抗菌化合物的基因的存在，这可能增强EMM - 1作为玉米植物生物防治剂的潜在用途。
