Dai Mengdi, Tan Xiangfeng, Chen Xuting, Cai Kangfeng, Zhong Yuanxiang, Ye Ziran, Kong Dedong
Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Key Laboratory of Digital Dry Land Crops of Zhejiang Province, Hangzhou, China.
Front Microbiol. 2024 Aug 5;15:1437799. doi: 10.3389/fmicb.2024.1437799. eCollection 2024.
The forage grass factory could break through the restrictions of land resources, region and climate to achieve efficient production throughout the year by accurate and intelligent management. However, due to its closed environment, mold outbreaks in the forage grass factory were severe, significantly affecting barley production. In this study, 9 contaminated barley tissues were collected and 45 strains were isolated in forage grass factory. After ITS sequencing, 45 strains were all identified as . Through stress factor assays, growth was seriously hindered by low concentration of sodium nitrate, high pH value and ozone water treatment. High pH and ozone water affected growth mainly by altering membrane integrity of . Sodium nitrate inhibited the growth of mainly by affecting the amount of sporulation. Low concentration of sodium nitrate and ozone water did not affect the growth of barley. High concentrations of sodium nitrate (100 mM) and pH values (8-8.5) inhibited barley growth. Among them, ozone water had the most obvious inhibition effect on . Large-scale ozone water treatment in the forage grass factory had also played a role in restoring barley production. Taken together, the green techonology to control mold disease and maintain the safety of forage through different physicochemical methods was selected, which was of considerable application value in animal husbandry.
牧草工厂可以突破土地资源、地域和气候的限制，通过精准智能管理实现全年高效生产。然而，由于其封闭环境，牧草工厂内霉菌爆发严重，显著影响大麦生产。本研究在牧草工厂采集了9份受污染的大麦组织，分离出45株菌株。经ITS测序，45株菌株均被鉴定为 。通过胁迫因子测定，低浓度硝酸钠、高pH值和臭氧水处理严重阻碍了 的生长。高pH值和臭氧水主要通过改变 的膜完整性来影响其生长。硝酸钠抑制 的生长主要是通过影响产孢量。低浓度硝酸钠和臭氧水不影响大麦生长。高浓度硝酸钠（100 mM）和pH值（8 - 8.5）抑制大麦生长。其中，臭氧水对 的抑制作用最为明显。牧草工厂内大规模臭氧水处理对恢复大麦生产也起到了作用。综上所述，选择了通过不同物理化学方法控制霉菌病害并维持牧草安全的绿色技术，这在畜牧业中具有相当大的应用价值。
