Sciorio Romualdo, Greco Pier Francesco, Tramontano Luca, Gullo Giuseppe, Greco Ermanno
Fertility Medicine and Gynaecological Endocrinology Unit, Department Woman Mother Child, Lausanne University Hospital, 1011 Lausanne, Switzerland.
Reproductive Medicine & IVF Unit, Royale Hayat Hospital, Hawalli 32002, Kuwait.
J Clin Med. 2025 Apr 19;14(8):2827. doi: 10.3390/jcm14082827.
: In assisted reproductive technology (ART), achieving a successful pregnancy requires optimizing an embryo culture and selecting the single embryo with the highest implantation potential, capable of resulting in a healthy pregnancy. The primary goal of this study was to determine the correlation between the blastocyst area and diameter and pregnancy outcomes in ART treatments. : In this study, the blastocyst diameter and area were measured to determine whether these morphometric features could predict pregnancy outcomes in couples undergoing ART with ICSI. This is a retrospective trial analyzing 665 patients who underwent an ART cycle with the transfer of a single blastocyst on day 5. : Both morphometric features assessed were significantly associated with implantation and ongoing pregnancy outcomes. Our results showed that the implantation rate (IR) and ongoing clinical pregnancy rate (CPR) were significantly higher with a blastocyst area ≥ 25,000 µm compared to <25,000 µm (IR: 69.8% versus 47.9%, < 0.001; CPR: 65.5% versus 45.9% < 0.001). Additionally, a blastocyst diameter ≥ 170 µm resulted in a significantly higher IR and CPR compared to embryos with a diameter < 170 µm (IR: 68.8% versus 36.6%, < 0.001; CPR: 66.3 versus 35.7%, < 0.001). : Blastocyst morphometric variables, being objective and measurable, are not subject to intra-operator variability and may serve as promising predictors of embryo viability and ongoing pregnancy success. These morphometric assessments could assist embryologists in selecting the embryo with the highest implantation potential from a cohort, as well as identifying those with a reduced chance of generating a successful pregnancy.
在辅助生殖技术（ART）中，要实现成功妊娠，需要优化胚胎培养条件并选择具有最高着床潜力的单个胚胎，以实现健康妊娠。本研究的主要目的是确定囊胚面积和直径与ART治疗中妊娠结局之间的相关性。
在本研究中，测量了囊胚直径和面积，以确定这些形态特征是否可以预测接受ICSI的ART夫妇的妊娠结局。这是一项回顾性试验，分析了665例在第5天进行单个囊胚移植的ART周期患者。
评估的两个形态特征均与着床和持续妊娠结局显著相关。我们的结果表明，与面积<25,000 µm²的囊胚相比，面积≥25,000 µm²的囊胚着床率（IR）和持续临床妊娠率（CPR）显著更高（IR：69.8% 对47.9%，<0.001；CPR：65.5% 对45.9%，<0.001）。此外，与直径<170 µm的胚胎相比，直径≥170 µm的囊胚导致IR和CPR显著更高（IR：68.8% 对36.6%，<0.001；CPR：66.3% 对35.7%，<0.001）。
囊胚形态计量学变量客观且可测量，不受操作者内部变异性的影响，可能是胚胎活力和持续妊娠成功的有前景的预测指标。这些形态学评估可以帮助胚胎学家从一组胚胎中选择具有最高着床潜力的胚胎，并识别那些成功妊娠机会降低的胚胎。
