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用于台式临床化学的二维离心法。

Two-dimensional centrifugation for desk-top clinical chemistry.

作者信息

Schultz S G, Holen J T, Donohue J P, Francoeur T A

出版信息

Clin Chem. 1985 Sep;31(9):1457-63.

PMID:4028394
Abstract

We have developed a new system for clinical chemistry analysis, the Vision System, in which centrifugal force is used to separate whole blood, measure reagent and plasma volumes, and complete all steps required for a spectrophotometric analysis. The system is based on use of a multichambered plastic test pack containing liquid reagents, which can be centrifuged at 500 X g in two planes, oriented at right angles to each other. Alternating centrifugal fields allows liquid reagents and plasma to flow into highly precise measuring and mixing chambers. A unique flash lamp and diode array spectrometer provide for optical measurements of 10 test packs at as many as eight wavelengths simultaneously. The temperature of each individual test pack is controlled by using a flash lamp coupled to a liquid crystal temperature sensor. Microprocessor control allows as many as 10 different chemistry reactions to be measured simultaneously on whole-blood, plasma, or serum samples. Comparison with results by an established batch-photometric analyzer demonstrated excellent precision and accuracy for various clinical chemistry tests.

摘要

我们开发了一种用于临床化学分析的新系统——视觉系统,该系统利用离心力分离全血、测量试剂和血浆体积,并完成分光光度分析所需的所有步骤。该系统基于使用一种含有液体试剂的多腔塑料测试包,该测试包可在两个相互垂直的平面上以500×g的离心力进行离心。交替的离心场使液体试剂和血浆流入高精度的测量和混合腔室。独特的闪光灯和二极管阵列光谱仪可同时在多达八个波长下对10个测试包进行光学测量。每个单独测试包的温度通过与液晶温度传感器相连的闪光灯进行控制。微处理器控制可同时对全血、血浆或血清样本进行多达10种不同的化学反应测量。与已建立的批量光度分析仪的结果进行比较,结果表明该系统在各种临床化学测试中具有出色的精密度和准确性。

相似文献

1
Two-dimensional centrifugation for desk-top clinical chemistry.用于台式临床化学的二维离心法。
Clin Chem. 1985 Sep;31(9):1457-63.
2
The application of two-dimensional centrifugation to clinical chemistry testing.二维离心技术在临床化学检测中的应用。
Ups J Med Sci. 1986;91(2):151-4. doi: 10.3109/03009738609178506.
3
Liquid-phase reactions started by rehydrating lyophilized reagents in a centrifugal analyzer.通过在离心分析仪中复水冻干试剂引发的液相反应。
Clin Chem. 1985 Sep;31(9):1506-8.
4
Centrifugal ion-selective electrode system for potassium in whole blood.用于全血中钾离子检测的离心式离子选择电极系统。
Clin Chem. 1990 Dec;36(12):2126-30.
5
Centrifugal microfluidic platforms: advanced unit operations and applications.离心微流控平台:高级单元操作及应用。
Chem Soc Rev. 2015 Oct 7;44(17):6187-229. doi: 10.1039/c4cs00371c. Epub 2015 Jun 2.
6
Development of a miniature fast analyzer.微型快速分析仪的研制
Clin Chem. 1972 Aug;18(8):753-61.
7
Optimization of EMIT reagent system using a COBAS-BIO centrifugal analyzer.
J Anal Toxicol. 1981 Sep-Oct;5(5):249-52. doi: 10.1093/jat/5.5.249.
8
Optimization and analytical applications of the technique dynamic introduction of liquids into centrifugal analyzers.
Clin Chem. 1974 Aug;20(8):932-41.
9
Portable simultaneous multiple analyte whole-blood analyzer for point-of-care testing.用于即时检验的便携式同步多分析物全血分析仪。
Clin Chem. 1992 Sep;38(9):1665-70.
10
A clinical evaluation of the Cobas Fara clinical chemistry analyzer for some routine serum enzymes and glucose.对Cobas Fara临床化学分析仪检测某些常规血清酶和葡萄糖的临床评估。
Am J Clin Pathol. 1987 Nov;88(5):603-9. doi: 10.1093/ajcp/88.5.603.

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