Bouzana Amina, Chekroud Zohra, Becheker Imène, Kamah Fatima, Sakhraoui Nora, Bensouici Chawki, Boufahja Fehmi, Alsalamah Sulaiman A, Alghonaim Mohammed I, Garzoli Stefania, Bendif Hamdi
Laboratory of Interactions, Biodiversity, Ecosystems and Biotechnology, Department of Nature and Life Sciences, Faculty of Sciences, University 20 August 1955 Skikda, Skikda 21000, Algeria.
Laboratory of Interactions, Biodiversity, Ecosystems and Biotechnology, Department of Ecology and Environment, Faculty of Sciences, University 20 August 1955 Skikda, Skikda 21000, Algeria.
Pharmaceuticals (Basel). 2025 Apr 16;18(4):578. doi: 10.3390/ph18040578.
: This study provides the first comprehensive phytochemical composition and biological evaluation of subsp. (Batt.) Greuter & Burdet, a plant endemic to Algeria with unexplored pharmacological potential. The objective is to identify novel bioactive metabolites in the plant's extracts and assess their potential applications for skincare and metabolic disorder management, addressing gaps in the current understanding of its medicinal value. : Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) profiling was used to analyze the hydromethanolic (HMeOH) leaf extract and identify bioactive compounds. The biological activities of HMeOH, ethyl acetate (EtOAc), and butanolic (n-BuOH) extracts were tested for cytotoxicity using the brine shrimp lethality test, photoprotective potential by calculating the sun protection factor (SPF), and enzymatic inhibitory activities against alpha-amylase, urease, and tyrosinase. : The LC-ESI-MS/MS profiling of the MeOH extract identified 22 bioactive compounds, including phenolic acids and flavonoids, some of which have not been previously reported in this species. Cytotoxicity tests showed that all extracts were non-toxic (half-lethal concentration (LC) > 100 micrograms per milliliter). The SPF values indicated significant photoprotective potential, with EtOAc (SPF = 45.19 ± 0.73) and n-BuOH (SPF = 43.81 ± 0.59) extracts showing high sun protection activity. The n-BuOH extract exhibited strong alpha-amylase inhibitory activity (half-maximal inhibitory concentration (IC) = 307.08 micrograms per milliliter), surpassing the standard acarbose (IC = 3650.93 micrograms per milliliter), suggesting potential applications in diabetes management. : subsp. demonstrates significant pharmacological potential as a source of bioactive secondary metabolites for skincare and metabolic disorder management. These findings provide new insights into the plant's therapeutic potential and set a foundation for future pharmacological and clinical investigations.
本研究首次对阿尔及利亚特有的具有未开发药理潜力的植物亚种（Batt.）Greuter & Burdet进行了全面的植物化学成分分析和生物学评价。目的是在该植物提取物中鉴定新型生物活性代谢物，并评估其在皮肤护理和代谢紊乱管理方面的潜在应用，以填补目前对其药用价值认识的空白。采用液相色谱-电喷雾电离串联质谱（LC-ESI-MS/MS）分析方法对水甲醇（HMeOH）叶提取物进行分析，以鉴定生物活性化合物。使用卤虫致死试验测试HMeOH、乙酸乙酯（EtOAc）和正丁醇（n-BuOH）提取物的细胞毒性，通过计算防晒系数（SPF）测试其光保护潜力，并测试其对α-淀粉酶、脲酶和酪氨酸酶的酶抑制活性。甲醇提取物的LC-ESI-MS/MS分析鉴定出22种生物活性化合物，包括酚酸和黄酮类化合物，其中一些在该物种中此前尚未有报道。细胞毒性测试表明，所有提取物均无毒（半数致死浓度（LC）>100微克/毫升）。SPF值表明具有显著的光保护潜力，乙酸乙酯提取物（SPF = 45.19 ± 0.73）和正丁醇提取物（SPF = 43.81 ± 0.59）显示出较高的防晒活性。正丁醇提取物表现出较强的α-淀粉酶抑制活性（半数最大抑制浓度（IC） = 307.08微克/毫升），超过标准阿卡波糖（IC = 三千六百五十点九三微克/毫升）,表明其在糖尿病管理方面的潜在应用。该植物亚种作为皮肤护理和代谢紊乱管理的生物活性次生代谢物来源，显示出显著的药理潜力。这些发现为该植物的治疗潜力提供了新的见解，并为未来的药理和临床研究奠定了基础。
