Zhao Yiru, Rao Shupei, Dai Guoli, Chen Jinhuan
College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing 100083, China.
Plants (Basel). 2025 Apr 8;14(8):1152. doi: 10.3390/plants14081152.
The gene family, a plant-specific subfamily of cytochrome P450 enzymes, plays pivotal roles in plant growth, development, and stress responses. Although the genes in many plants have been widely studied, little is known about them in . In this study, we identified six genes in both and through comprehensive bioinformatics analysis. These genes exhibited high conservation in protein structure, gene organization, and conserved motifs. Phylogenetic analysis revealed they are close in terms of homology to genes in , tomato, and eggplant. -acting element analysis of the promoter regions indicated that genes are involved in light, hormone, and stress responses, with tissue-specific expression patterns observed across different developmental stages. Subcellular localization experiments confirmed that LrCYP78A5 is localized in the endoplasmic reticulum. Overexpression of in resulted in a significant increase in chlorophyll content, indicating the former's potential role in plant growth. These findings provide valuable insights into the functional roles of the gene family in goji, highlighting their potential involvement in growth regulation and metabolic processes.
该基因家族是细胞色素P450酶的植物特异性亚家族，在植物生长、发育和应激反应中起关键作用。尽管许多植物中的该基因已得到广泛研究，但在枸杞中对其了解甚少。在本研究中，我们通过全面的生物信息学分析在枸杞和宁夏枸杞中鉴定出六个该基因。这些基因在蛋白质结构、基因组织和保守基序方面表现出高度保守性。系统发育分析表明，它们在同源性上与烟草、番茄和茄子中的该基因相近。对启动子区域的顺式作用元件分析表明，该基因参与光、激素和应激反应，在不同发育阶段观察到组织特异性表达模式。亚细胞定位实验证实LrCYP78A5定位于内质网。在烟草中过表达该基因导致叶绿素含量显著增加，表明其在植物生长中的潜在作用。这些发现为枸杞中该基因家族的功能作用提供了有价值的见解，突出了它们在生长调节和代谢过程中的潜在参与。
