Genome-Wide Identification of the Gene Family in and Functional Characterization of .

The gene family, a plant-specific subfamily of cytochrome P450 enzymes, plays pivotal roles in plant growth, development, and stress responses. Although the genes in many plants have been widely studied, little is known about them in . In this study, we identified six genes in both and through comprehensive bioinformatics analysis. These genes exhibited high conservation in protein structure, gene organization, and conserved motifs. Phylogenetic analysis revealed they are close in terms of homology to genes in , tomato, and eggplant. -acting element analysis of the promoter regions indicated that genes are involved in light, hormone, and stress responses, with tissue-specific expression patterns observed across different developmental stages. Subcellular localization experiments confirmed that LrCYP78A5 is localized in the endoplasmic reticulum. Overexpression of in resulted in a significant increase in chlorophyll content, indicating the former's potential role in plant growth. These findings provide valuable insights into the functional roles of the gene family in goji, highlighting their potential involvement in growth regulation and metabolic processes.