Atrous Ghofrane, Piqueras Abel, Diaz-Vivancos Pedro, Hernández-Cánovas Ana, Hamed Karim Ben, Hernández José A, Barba-Espín Gregorio
Group of Fruit Trees Biotechnology, Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, 30100 Murcia, Spain.
Centre of Biotechnology of Borj Cedria, Laboratory of Extremophile Plants, BP 95, Hammam-Lif 2050, Tunisia.
Plants (Basel). 2025 Apr 9;14(8):1164. doi: 10.3390/plants14081164.
Halophytes hold significance for soil desalination and co-cultivation in farming systems. A major impediment to their use is the standardization of their performance, since halophytes are mainly wild plants, in addition to the need for a constant supply of the most suitable species. In this work, using highly salt-tolerant clones of obtained previously from in vitro micropropagation and selection, we compared the physiological and biochemical responses of these clones and their wild counterparts to high salinity levels (428 mM NaCl) under glasshouse conditions. In vitro-derived clones displayed a superior biomass production (27%) and higher chloride concentration in the shoot (28%), compared to the wild plants. On the other hand, wild specimens showed more stress symptoms and a less efficient photosynthesis, which was correlated with higher levels of oxidative stress and with a remarkable induction of peroxidase activity. Therefore, a higher incidence of salinity-related oxidative stress in the wild halophytes in comparison to the clones is concluded. This represents the first ex vitro evaluation of halophyte clones selected by means of micropropagation and provides insights into the salinity tolerance mechanisms of .
盐生植物对于土壤脱盐和农业系统中的间作具有重要意义。其应用的一个主要障碍是性能的标准化，因为盐生植物主要是野生植物，此外还需要持续供应最合适的物种。在这项工作中，我们使用先前通过离体微繁殖和筛选获得的高度耐盐克隆，比较了这些克隆及其野生对应物在温室条件下对高盐度水平（428 mM NaCl）的生理和生化反应。与野生植物相比，离体衍生的克隆表现出更高的生物量产量（27%）和地上部更高的氯离子浓度（28%）。另一方面，野生植株表现出更多的胁迫症状和较低的光合作用效率，这与较高水平的氧化胁迫以及过氧化物酶活性的显著诱导相关。因此，可以得出结论，与克隆相比，野生盐生植物中与盐度相关的氧化胁迫发生率更高。这是对通过微繁殖选择的盐生植物克隆进行的首次离体评估，并为[具体植物名称]的耐盐机制提供了见解。
