Benmouna Manal, Benammar Chahid, Khan Amira Sayed, Djeziri Fatima Zohra, Hichami Aziz, Khan Naim A
Physiologie de Nutrition & Toxicology (NUTox), UMR UB/INSERM 1231 Center for Cellular & Translational Molecular Medicine (CTM), Université Bourgogne Europe, & FCS Bourgogne-Franche Comté, LipSTIC LabEx, 21000 Dijon, France.
Laboratoire des Produits Naturels (LAPRONA), Université Abou Bekr Belkaid, Tlemcen 13000, Algeria.
Nutrients. 2025 Apr 9;17(8):1308. doi: 10.3390/nu17081308.
Obesity is associated with the altered gustatory perception of dietary fatty acids. Celastrol, a triterpene, has been demonstrated to exert anti-obesity effects in rodents. We assessed the role of Celastrol in the modulation of the oro-sensory perception of lipids in control and high-fat diet (HFD)-induced obese mice.
Male mice of the C57B/6J strain were fed a HFD for 11 weeks and then were administered or not with Celastrol further for 4 weeks. The body weight was recorded weekly. Before the sacrifice, the animals were subjected to oro-sensory detection of a dietary long-chain fatty acid in a two-bottle choice paradigm. After the sacrifice, the fungiform taste buds were isolated and analyzed for mRNA expression, encoding fat sensors (CD36 and GPR120) and pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α). Circulating concentrations of IL-6 and TNF-α were also determined, and liver was used to analyze the mRNA expression of lipogenic genes.
Celastrol administration in obese mice decreased body weight and also re-established the loss of oro-sensory perception for a dietary fatty acid, and this phenomenon was, in part, due to the upregulation of mRNA, encoding fat taste receptors (CD36 and GPR120) in tongue taste bud cells. Furthermore, Celastrol decreased inflammation both in taste buds and blood circulation.
Our findings suggest that Celastrol decreases body weight gain, ameliorates the gustatory perception of lipids, and downregulates inflammation in obese mice.
肥胖与膳食脂肪酸味觉感知改变有关。三萜类化合物雷公藤红素已被证明在啮齿动物中具有抗肥胖作用。我们评估了雷公藤红素在调节对照小鼠和高脂饮食（HFD）诱导的肥胖小鼠对脂质的口腔感觉感知中的作用。
将C57B/6J品系雄性小鼠喂食高脂饮食11周，然后再给予或不给予雷公藤红素4周。每周记录体重。在处死前，在双瓶选择范式中对动物进行膳食长链脂肪酸的口腔感觉检测。处死动物后，分离菌状味蕾并分析编码脂肪传感器（CD36和GPR120）和促炎细胞因子（IL-1β、IL-6和TNF-α）的mRNA表达。还测定了循环中IL-6和TNF-α的浓度，并使用肝脏分析脂肪生成基因的mRNA表达。
给肥胖小鼠施用雷公藤红素可降低体重，并重新建立对膳食脂肪酸的口腔感觉感知丧失，这种现象部分归因于舌味蕾细胞中编码脂肪味觉受体（CD36和GPR120）的mRNA上调。此外，雷公藤红素可减轻味蕾和血液循环中的炎症。
我们的研究结果表明，雷公藤红素可减少肥胖小鼠的体重增加，改善脂质的味觉感知，并下调炎症反应。
