Laboratoire des Produits Naturels, Université Abou-Bekr Belkaïd, Tlemcen, 13000, Algeria.
Physiologie de la Nutrition & Toxicologie, UMR U1231 INSERM/Université de Bourgogne-Franche Compté (UBFC), Dijon, 21000, France.
Biochimie. 2018 Sep;152:110-120. doi: 10.1016/j.biochi.2018.06.025. Epub 2018 Jun 30.
Obesity, triggered by high-fat diet (HFD), is associated to altered gustatory perception of dietary lipids. Oleanolic acid (OLA), a triterpene, has been reported to exert anti-obesity effects in animal models. Hence, we investigated the role of OLA in the modulation of oro-sensory perception of lipids in control and HFD-induced obese mice. As expected, OLA-treated obese mice exhibited a decrease in body, liver, and visceral adipose tissue weights. OLA treatment improved glucose tolerance, insulin level, plasma lipopolysaccharide (LPS), and hepatic cholesterol and triglyceride concentrations. OLA-treated obese mice exhibited higher fat preference compared to untreated obese mice, probably due to the increase in mRNA encoding CD36, a fat taste receptor, in mouse taste bud cells (mTBC). This phenomenon was associated with fatty-acid induced increases in free intracellular calcium concentrations, [Ca]i, induced in mTBC from OLA-treated obese mice. OLA also influenced the expression of mRNA encoding pro-inflammatory cytokines (IL-1β and IL-6) and some lipogenic genes (PPARα, SREBP1, FAS, ChREBP, and G6Pase) in liver and adipose tissue. These findings reveal that OLA improves gustatory perception of lipids and exerts protective effects in obesity.
肥胖是由高脂肪饮食（HFD）引起的，与膳食脂质味觉感知改变有关。齐墩果酸（OLA）是一种三萜，已被报道在动物模型中具有抗肥胖作用。因此，我们研究了 OLA 在调节对照和 HFD 诱导肥胖小鼠口腔感知脂质中的作用。正如预期的那样，OLA 治疗的肥胖小鼠的体重、肝脏和内脏脂肪组织重量均降低。OLA 治疗可改善葡萄糖耐量、胰岛素水平、血浆脂多糖（LPS）和肝胆固醇及甘油三酯浓度。与未治疗的肥胖小鼠相比，OLA 治疗的肥胖小鼠表现出更高的脂肪偏好，这可能是由于小鼠味蕾细胞（mTBC）中编码脂肪味觉受体 CD36 的 mRNA 增加所致。这种现象与脂肪酸诱导的 mTBC 中游离细胞内钙浓度 ([Ca]i) 增加有关，而 OLA 治疗的肥胖小鼠中游离细胞内钙浓度 ([Ca]i) 增加。OLA 还影响肝脏和脂肪组织中编码促炎细胞因子（IL-1β 和 IL-6）和一些脂生成基因（PPARα、SREBP1、FAS、ChREBP 和 G6Pase）的 mRNA 表达。这些发现表明 OLA 可改善脂质味觉感知，并在肥胖中发挥保护作用。
