McMahon K E, Farrell P M
Clin Chim Acta. 1985 Jun 30;149(1):1-12. doi: 10.1016/0009-8981(85)90267-0.
The development of a micropyrolysis gas chromatographic technique for the determination of free choline in plasma and erythrocytes is reported as a means to clinically assess choline status. A micropyrolyzer syringe unit was fabricated and the method was standardized through repeated trials with a single plasma specimen. The interassay coefficient of variance was 1.3% for the standardized trials on plasma and erythrocytes. Choline status was assessed in control women, mothers and premature infants at birth and up to 14 days of life. At birth, plasma choline levels in the infants (32.2 +/- 5.5 mumol/l) were significantly higher than those in the mothers (12.9 +/- 2.6 mumol/l) and the control women (16.9 +/- 1.6 mumol/l). The infant's plasma choline concentrations had decreased significantly by 7 days of life and remained at the lower level at day 14, independent of the nutritional intervention administered.
本文报道了一种用于测定血浆和红细胞中游离胆碱的微热解气相色谱技术的开发，以此作为临床评估胆碱状态的一种手段。制作了一个微热解注射器单元，并通过对单个血浆样本进行反复试验使该方法标准化。血浆和红细胞标准化试验的批间变异系数为1.3%。对对照组女性、母亲以及早产儿出生时和出生后14天内的胆碱状态进行了评估。出生时，婴儿的血浆胆碱水平（32.2±5.5μmol/L）显著高于母亲（12.9±2.6μmol/L）和对照组女性（16.9±1.6μmol/L）。婴儿的血浆胆碱浓度在出生7天时显著下降，并在第14天维持在较低水平，与所给予的营养干预无关。
