采用简单的放射酶法分析人血浆中的游离胆碱：年龄分布及进餐的影响。

Free choline in human plasma analysed by simple radio-enzymatic procedure: age distribution and effect of a meal.

作者信息

Eckernäs S A, Aquilonius S M

出版信息

Scand J Clin Lab Invest. 1977 Apr;37(2):183-7. doi: 10.1080/00365517709156075.

Abstract

Ultrafiltration of plasma was shown to be a simple and rapid method to obtain a stable sample for direct measurement of free choline (Ch) in plasma by a radioenzymatic procedure. Free Ch was analysed in plasma from healthy volunteers fasted 12-15 h and 1 h after a meal. The free Ch concentration was found within narrow limits with a mean of 10.6 +/- 0.4 mumol/l in the fasted subjects and 11.5 +/- 0.3 mumol/l 1 h after a meal. The difference is significant (paired t test, P less than 0.01, n = 23). Dietary influence on the free Ch concentration in human plasma is suggested. In three newborn infants (1-3 min post partum) the Ch concentration in plasma from the umbilical vein was 24.5 +/- 1.9 mumol/l.

摘要

血浆超滤被证明是一种简单快速的方法，可通过放射酶法直接测量血浆中游离胆碱（Ch）获得稳定样本。对禁食12 - 15小时的健康志愿者以及进食1小时后的血浆中的游离Ch进行了分析。在禁食受试者中，游离Ch浓度在狭窄范围内，平均值为10.6±0.4μmol/L，进食1小时后为11.5±0.3μmol/L。差异具有统计学意义（配对t检验，P<0.01，n = 23）。提示饮食对人体血浆中游离Ch浓度有影响。在三名新生儿（产后1 - 3分钟）中，脐静脉血浆中的Ch浓度为24.5±1.9μmol/L。