Dveyrin Zeev, Alon Tal, Makhon Andrei, Nissan Israel, Mor Zohar, Rorman Efrat
National Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv 6810416, Israel.
Central District Department of Health, Ministry of Health, Ramla 7243003, Israel.
Microorganisms. 2025 Mar 26;13(4):750. doi: 10.3390/microorganisms13040750.
(NG) is a globally significant sexually transmitted infection (STI) with increasing antimicrobial resistance (AMR), posing a serious threat to public health. Between 2016 and 2022, the Israeli National NG Reference Center (INNGRC) comprehensively analyzed NG isolates in Israel to determine AMR patterns and sequence types (STs). Antimicrobial susceptibility testing (AST) was performed on 1205 NG isolates using E-test gradient strips, and NG-MAST analysis was conducted on 279 isolates via Sanger sequencing and whole genome sequencing (WGS). Surveillance revealed high resistance rates to ciprofloxacin (54.4%), azithromycin (41.3%), tetracycline, and benzylpenicillin, while all isolates remained susceptible to ceftriaxone and spectinomycin. Multi-drug resistance (MDR) was observed in 8.6% of isolates, and 3% were classified as extensively drug-resistant (XDR). NG-MAST analysis identified 72 distinct STs, with ST292, ST4269, and ST5441 being the most prevalent. ST19665 and ST11461 predominated in 2022, while ST292, ST5441, and ST16169 were more abundant in 2018. The findings highlight the increasing prevalence of AMR in NG in Israel and underscore the importance of continuous surveillance and molecular characterization by reference laboratories like the INNGRC to inform treatment strategies and public health interventions, ultimately reducing the burden of this critical STI.
淋病（NG）是一种在全球范围内具有重要意义的性传播感染（STI），其抗菌药物耐药性（AMR）不断增加，对公共卫生构成严重威胁。2016年至2022年期间，以色列国家淋病参考中心（INNGRC）对以色列的淋病分离株进行了全面分析，以确定AMR模式和序列类型（STs）。使用E-test梯度条对1205株淋病分离株进行了抗菌药物敏感性测试（AST），并通过桑格测序和全基因组测序（WGS）对279株分离株进行了淋病多重抗原序列分型（NG-MAST）分析。监测显示，对环丙沙星（54.4%）、阿奇霉素（41.3%）、四环素和苄青霉素的耐药率很高，而所有分离株对头孢曲松和大观霉素仍敏感。在8.6%的分离株中观察到多重耐药（MDR），3%被归类为广泛耐药（XDR）。NG-MAST分析确定了72种不同的STs，其中ST292、ST4269和ST5441最为普遍。ST19665和ST11461在2022年占主导地位，而ST292、ST5441和ST16169在2018年更为常见。这些发现突出了以色列淋病中AMR患病率的上升，并强调了像INNGRC这样的参考实验室进行持续监测和分子特征分析的重要性，以为治疗策略和公共卫生干预提供信息，最终减轻这种关键STI的负担。
