Stakelienė Violeta, Pašakinskienė Izolda, Matijošiūtė Saulė, Martūnas Justas, Štukėnienė Gitana
Botanical Garden, Vilnius University, Kairėnų 43, 10239 Vilnius, Lithuania.
Life Sciences Centre, Vilnius University, Saulėtekio 7, 10221 Vilnius, Lithuania.
Microorganisms. 2025 Mar 31;13(4):799. doi: 10.3390/microorganisms13040799.
This study investigates the diversity and distribution of root endophyte fungi and bacteria across and grasses, including open-grassland and forest species. The species examined include perennials such as , . , . , , and . × . hybrids and the annuals . and . . A total of 21 fungal species (60 isolates) and 26 bacterial taxa (59 isolates) were recovered in the culture (PDA medium for fungi and LB for bacteria) from the root cuttings of these grasses. fungi and sp. bacteria were the most prevalent endophytes, with each being identified in five of the seven plant species examined. The annuals . and . exhibited a higher abundance of endophytes than that in their perennial relatives, suggesting the benefits of microbial associations in supporting their short life cycles. The woodland . demonstrated the highest fungal endophyte diversity, with six species identified. In contrast, the open-grassland perennials . , . , and . hosted only one to two species. Two Basidiomycota, and , were exclusively obtained from the roots of the forest grass . . Notably, the open-grassland perennial . exhibited the highest bacterial diversity, with nine species present. However, it showed the lowest fungal diversity, with only one species detected. Overall, our study reveals distinct patterns of fungal and bacterial endophyte diversity in the roots of and grasses, with variations linked to host species, growth type traits, and ecological adaptations. Among the root-derived endophytes isolated, several fungi and bacteria are potential candidates for plant growth promotion and biocontrol. Therefore, the findings of this study provide potential implications for improved grassland management and crop breeding strategies aimed at specific climate and/or soil conditions.
本研究调查了根茎内生真菌和细菌在多种禾本科植物中的多样性和分布情况，这些植物包括开阔草原和森林中的物种。所研究的物种包括多年生植物，如……、……、……、……、……以及……×……杂交种，还有一年生植物……和……。通过在培养基（真菌用PDA培养基，细菌用LB培养基）中对这些禾本科植物的根插条进行培养，共分离出21种真菌（60个分离株）和26个细菌分类单元（59个分离株）。……真菌和……种细菌是最普遍的内生菌，在所检测的7种植物中的5种植物中均有发现。一年生植物……和……的内生菌丰度高于其多年生亲缘植物，这表明微生物共生关系对支持它们的短生命周期有益。林地植物……的真菌内生菌多样性最高，鉴定出了6个物种。相比之下，开阔草原多年生植物……、……和……仅含有一到两个物种。两种担子菌……和……仅从森林禾本科植物……的根部获得。值得注意的是，开阔草原多年生植物……的细菌多样性最高，有9个物种；然而，其真菌多样性最低，仅检测到1个物种。总体而言，我们的研究揭示了禾本科植物根部真菌和细菌内生菌多样性的不同模式，其变化与宿主物种、生长类型特征和生态适应性有关。在所分离的源自根部的内生菌中，有几种真菌和细菌是促进植物生长和生物防治的潜在候选者。因此，本研究结果对于针对特定气候和/或土壤条件改进草地管理和作物育种策略具有潜在意义。
