Wirsel Stefan G R, Leibinger Wolfgang, Ernst Michael, Mendgen Kurt
Lehrstuhl für Phytopathologie, Fachbereich für Biologie, Universität Konstanz, Universitätstr. 10, D-78434 Konstanz, Germany.
New Phytol. 2001 Mar;149(3):589-598. doi: 10.1046/j.1469-8137.2001.00038.x.
• Variation in endophytic fungal diversity closely associated with roots, stems and leaves of common reed (Phragmites australis) is reported here at sites with different oxygen conditions. • Fungi isolated from surface-sterilized reed tissue were identified and characterized by morphological and molecular methods including internal transcribed spacer (ITS) sequence analysis from two dry and two flooded sites at Lake Constance (Germany). • Most isolates were ascomycetes, some basidiomycetes. There were differences in distribution between dry and flooded sites. Trichoderma sp. and Cylindrocarpon sp. were almost exclusively recovered from roots of reed growing at dry sites, whereas Microdochium sp. and Cladosporum sp. were more frequently found at flooded sites. The preference of Trichoderma sp. for drier sites was confirmed by a nested PCR assay targeting the variable ITS region. • A diverse assemblage of endophytic fungi that differ in distribution between aerated and nonaerated soils is found in reed habitats. The rich mycoflora associated with roots in completely anaerobic soils might depend on downward oxygen transport via an aerenchyma-based ventilation system.
• 本文报道了在不同氧气条件下，与芦苇（Phragmites australis）根、茎和叶紧密相关的内生真菌多样性变化。
• 从表面消毒的芦苇组织中分离出的真菌，通过形态学和分子方法进行鉴定和表征，包括对来自德国康斯坦茨湖两个干燥地点和两个水淹地点的内部转录间隔区（ITS）序列分析。
• 大多数分离株为子囊菌，一些为担子菌。干燥地点和水淹地点的分布存在差异。木霉属（Trichoderma sp.）和柱孢属（Cylindrocarpon sp.）几乎仅从生长在干燥地点的芦苇根中分离得到，而微座孢属（Microdochium sp.）和枝孢属（Cladosporum sp.）在水淹地点更常见。针对可变ITS区域的巢式PCR分析证实了木霉属对较干燥地点的偏好。
• 在芦苇栖息地发现了不同的内生真菌组合，它们在通气土壤和非通气土壤中的分布不同。与完全厌氧土壤中的根相关的丰富真菌区系可能依赖于通过基于通气组织的通气系统向下输送氧气。
