Cunha Nathan Mickael de Bessa, Teixeira Itamar Rosa, Teixeira Gisele Carneiro da Silva, Rocha Ednaldo Cândido, Bravo Tamires Ester Peixoto, Souza Andressa Laís Caldeira de, Damião Eulina Fernandes, Sbroggio Filho Alexandre Marcos
Institute of Agricultural Science, State University of Goiás, Anápolis 75132-903, GO, Brazil.
Department of Agronomy, Federal University of Goiás, Goiânia 74690-900, GO, Brazil.
Microorganisms. 2025 Apr 1;13(4):805. doi: 10.3390/microorganisms13040805.
This study evaluates the impact of reinoculation and co-inoculation at different growth stages on the physiological quality of common bean seeds. A randomized block design was used, assessing germination, vigor, electrical conductivity, seedling length, and dry mass. Treatments T7 (co-inoculation + at R5) showed the highest germination rates, indicating enhanced seed viability. The accelerated aging test revealed that T7 exhibited greater resistance to stress, presenting greater seedling vigor, whereas T10 and T11 were more susceptible. The electrical conductivity results remained stable across treatments, suggesting that cell membrane integrity was not significantly compromised. Seedling length and dry mass did not present significant variations, reinforcing the idea that early germination and vigor are primary indicators of seed quality. Canonical discriminant analysis and MANOVA confirmed significant treatment differences, highlighting the influence of inoculation strategies on seed physiology. Overall, co-inoculation with and (particularly in T7) demonstrated potential to improve seed quality at lower cost, offering sustainable alternatives for optimizing agricultural production.
本研究评估了在不同生长阶段进行再接种和共接种对普通菜豆种子生理质量的影响。采用随机区组设计，评估发芽率、活力、电导率、幼苗长度和干质量。处理T7（共接种 + 在R5阶段）显示出最高的发芽率，表明种子活力增强。加速老化试验表明，T7对胁迫具有更强的抗性，表现出更高的幼苗活力，而T10和T11更易受影响。各处理的电导率结果保持稳定，表明细胞膜完整性未受到显著损害。幼苗长度和干质量没有显著差异，这进一步证明了早期发芽和活力是种子质量的主要指标。典型判别分析和多变量方差分析证实了处理间存在显著差异，突出了接种策略对种子生理的影响。总体而言，与[未提及具体接种物]共接种（特别是在T7中）显示出以较低成本提高种子质量的潜力，为优化农业生产提供了可持续的替代方案。
