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从墨西哥奈卡矿分离出的两株菌株的生理学、重金属抗性及基因组分析

Physiology, Heavy Metal Resistance, and Genome Analysis of Two Strains Isolated from the Naica Mine (Mexico).

作者信息

González-Sánchez Antonio, Lozano-Aguirre Luis, Jiménez-Flores Guadalupe, López-Sámano Mariana, García-de Los Santos Alejandro, Cevallos Miguel A, Le Borgne Sylvie

机构信息

Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Unidad Cuajimalpa, Ciudad de México 05348, Mexico.

Unidad de Análisis Bioinformáticos, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca 62210, Mexico.

出版信息

Microorganisms. 2025 Apr 2;13(4):809. doi: 10.3390/microorganisms13040809.

DOI:10.3390/microorganisms13040809
PMID:40284645
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12029693/
Abstract

Here, we report the characterization of two strains, NOV2-1 and OV2-1, isolated from an iron-oxide deposit in an underground tunnel of the Naica mine in Mexico. This unique biotope, characterized by its high temperature (≈50 °C) and the presence of heavy metals, is no longer available for sampling at this time. The genomes of NOV2-1 and OV2-1 comprised two replicons: a chromosome of 3.58 and 3.53 Mb, respectively, and a chromid of 2.1 Mb in both strains. No plasmids were found. The average nucleotide identity and the core genome phylogeny showed that NOV2-1 and OV2-1 belonged to the species. NOV2-1 and OV2-1 grew up to 48 °C, with an optimal temperature of 42 °C. Discrete differences were observed between CCUG38401, NOV2-1, and OV2-1 in the biochemical tests. NOV2-1 and OV2-1 presented resistance to zinc, lead, copper, cadmium, nickel, and cobalt. Several complete and incomplete gene clusters related to the resistance to these heavy metals (, , 1, - 2, , , and ) were detected in the genome of these strains. Although further studies are needed to determine the origin and role of the detected gene clusters, it is suggested that the system may have been mobilized by horizontal gene transfer. This study expands the extreme biotopes where strains can be retrieved.

摘要

在此，我们报告了从墨西哥奈卡矿地下隧道的铁氧化物矿床中分离出的两株菌株NOV2-1和OV2-1的特征。这个独特的生物群落以其高温（约50°C）和重金属的存在为特征，目前已无法再进行采样。NOV2-1和OV2-1的基因组由两个复制子组成：两条染色体分别为3.58 Mb和3.53 Mb，两株菌株还有一个大小为2.1 Mb的染色体外遗传因子。未发现质粒。平均核苷酸同一性和核心基因组系统发育分析表明，NOV2-1和OV2-1属于同一物种。NOV2-1和OV2-1能在高达48°C的温度下生长，最适温度为42°C。在生化试验中观察到CCUG38401、NOV2-1和OV2-1之间存在明显差异。NOV2-1和OV2-1对锌、铅、铜、镉、镍和钴具有抗性。在这些菌株的基因组中检测到了几个与这些重金属抗性相关的完整和不完整基因簇（、、1、-2、、、和）。尽管需要进一步研究来确定检测到的基因簇的起源和作用，但有人认为该系统可能是通过水平基因转移而被激活的。这项研究扩展了可检索到菌株的极端生物群落范围。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/e4fd9b734f2f/microorganisms-13-00809-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/17c3a57c272e/microorganisms-13-00809-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/b02d16b35744/microorganisms-13-00809-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/83e55c117823/microorganisms-13-00809-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/181c1ad6750e/microorganisms-13-00809-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/5856f414fe5a/microorganisms-13-00809-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/626562eab3e2/microorganisms-13-00809-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/a611302e2f71/microorganisms-13-00809-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/e4fd9b734f2f/microorganisms-13-00809-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/17c3a57c272e/microorganisms-13-00809-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/b02d16b35744/microorganisms-13-00809-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/83e55c117823/microorganisms-13-00809-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/181c1ad6750e/microorganisms-13-00809-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/5856f414fe5a/microorganisms-13-00809-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/626562eab3e2/microorganisms-13-00809-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/a611302e2f71/microorganisms-13-00809-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4edf/12029693/e4fd9b734f2f/microorganisms-13-00809-g008.jpg

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