Microbiology Laboratory, Department of Biology, Faculty of Science and Technology State Islamic, University of Maulana Malik Ibrahim Malang, Indonesia, Jln Gajayana No.50, Malang, East Java 65144, Indonesia E-mail:
Water Sci Technol. 2020 May;81(10):2244-2249. doi: 10.2166/wst.2020.281.
Anthropogenic activities such as oil exploration have resulted in an environmental concern as they are comprised of residual hydrocarbons and metals. Following the hypothesis that endogenous bacterial communities have enhanced tolerance to heavy metals, we isolated and characterized culturable lead-resistant bacteria from an oil wastewater sample and determined whether they could reduce lead ions from the medium. The wastewater sample containing indigenous bacteria were taken out from a traditional oil field, Bojonegoro District, East Java, Indonesia, and bacteria were cultured Halomonas complex (HMC) medium containing lead (II) chloride (PbCl) with different concentrations. Bioaccumulation of lead by heavy-metals resistant bacteria was determined by using atomic absorption spectrophotometry (AAS). Our result found 21 bacterial strains that resist lead ions, of which one strain (RPb5-3) highly resisted to 10 mM. This bacterial strain also exhibited the highest accumulation of Pb, and it could grow at various temperatures, or more than their original environment. The bacterial strains could be used for bioremediation of lead toxicity, especially in oil pollutants.
人为活动，如石油勘探，引起了环境方面的关注，因为它们含有残余的碳氢化合物和金属。基于内源性细菌群落对重金属具有更强的耐受性的假设，我们从石油废水样本中分离并鉴定了可培养的耐铅细菌，并确定它们是否能从培养基中还原铅离子。从印度尼西亚东爪哇省博约内戈罗区的一个传统油田中取出含有土著细菌的废水样本，并在含有氯化铅（PbCl）的 Halomonas 复合（HMC）培养基中培养不同浓度的铅（II）。使用原子吸收分光光度法（AAS）测定耐重金属细菌对铅的生物累积。我们的结果发现了 21 株耐铅离子的细菌，其中一株（RPb5-3）对 10 mM 的铅具有高度抗性。该细菌株还表现出最高的 Pb 积累量，并且能够在各种温度下或超过其原始环境下生长。这些细菌株可用于生物修复铅毒性，特别是在石油污染物中。
