Pricemou Siba, Soropogui Barré, Bérété Fanta, Beavogui Michel Bossou, Samoura Aboubacar, Picardeau Mathieu, Bourhy Pascale, Tordo Noël, Grayo Solène
Biodiversity and One Health Group, Institut Pasteur de Guinée, Conakry BP 4416, Guinea.
Centre de Recherche en Virologie, Laboratoire des Fièvres Hémorragiques Virales de Guinée (CRV-LFHVG), Conakry BP 4416, Guinea.
Microorganisms. 2025 Apr 7;13(4):833. doi: 10.3390/microorganisms13040833.
Leptospirosis is a bacterial zoonosis caused by pathogenic species from the genus . Infection mostly occurs through indirect contact with environmental water contaminated with the urine of reservoir animals. Information on the circulation of leptospirosis in West Africa, as well as its potential reservoir hosts, is limited. Therefore, we carried out trapping surveys in the Guinean forest in November 2022, and samples were collected from 42 micromammals. The animals were both morphologically and genetically identified. The lungs and kidneys were screened for using -gene-targeting real-time PCR, and positive samples were genotyped based on the polymorphic gene. species were detected in the kidneys of three micromammals: , , and . was identified in and that were captured in two different villages. The phylogenetic analysis indicated that this subspecies had previously been detected in one patient in Mayotte, but the reservoir was not identified. A new subspecies of was isolated in from the same village as the positive for . The high diversity of both the reservoirs and species in the Guinean forest indicates that we should study other natural regions and reinforce communities' awareness of infection risks in Guinea.
钩端螺旋体病是一种由钩端螺旋体属致病物种引起的细菌性人畜共患病。感染主要通过间接接触被储存宿主动物尿液污染的环境水而发生。关于西非钩端螺旋体病的传播情况及其潜在储存宿主的信息有限。因此，我们于2022年11月在几内亚森林进行了诱捕调查，并从42只小型哺乳动物身上采集了样本。对这些动物进行了形态学和遗传学鉴定。使用靶向基因的实时PCR对肺和肾进行筛查，并根据多态性基因对阳性样本进行基因分型。在三只小型哺乳动物的肾脏中检测到钩端螺旋体物种：非洲沟鼠、黑家鼠和屋顶鼠。在两个不同村庄捕获的非洲沟鼠和黑家鼠中鉴定出了问号钩端螺旋体。系统发育分析表明，该亚种此前在马约特岛的一名患者中被检测到，但储存宿主未被确定。在与问号钩端螺旋体阳性的非洲沟鼠来自同一村庄的屋顶鼠中分离出了问号钩端螺旋体的一个新亚种。几内亚森林中储存宿主和钩端螺旋体物种的高度多样性表明，我们应该研究其他自然区域，并加强几内亚社区对钩端螺旋体感染风险的认识。
