Wen Tong-Yue, Xie Xing-Li, Kong Wei-Liang, Wu Xiao-Qin
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China.
Microorganisms. 2025 Apr 9;13(4):853. doi: 10.3390/microorganisms13040853.
Microorganisms exert antagonistic effects on pathogens through different mechanisms, thereby achieving biological control of plant diseases. Many strains can produce complex secondary metabolites and substances that have toxic effects on host cells. The phage tail-like bacteriocins (tailocins) is a compound with antibacterial activity. However, its function in has not yet been reported. This article explores the ability of WS-FJ9 to antagonise plant pathogenic fungi and oomycetes, screening the potential tailocins in the strain WS-FJ9 and verifying their function, to reveal its novel antimicrobial mechanisms. We found that WS-FJ9 had strong antagonistic effects on the plant pathogenic fungi and , and the pathogenic oomycete . The phage tail-like protein Bm_67459 was predicted from the WS-FJ9 strain genome. The Bm_67459 cDNA encoded 111 amino acid sequence, and the relative molecular weight was approximately 11.69 kDa, the theoretical isoelectric point (pI) was 5.49, and it was a hydrophilic protein. Bm_67459 had no transmembrane helix region or signal peptide, and it belonged to the Phage_TAC_7 super family. qRT-PCR results showed that gene expression was significantly upregulated during contact between WS-FJ9 and . The purified Bm_67459 protein significantly inhibited mycelial growth at 10 μg·mL. In summary, the WS-FJ9 strain had broad-spectrum anti-phytopathogenic activity, and the tailocin Bm_67459 was an important effector against the plant pathogen , which helps to reveal the antagonistic mechanism of this strain at the molecular level and provides excellent strain resources for the biological control of plant diseases.
微生物通过不同机制对病原体产生拮抗作用，从而实现对植物病害的生物防治。许多菌株能产生对宿主细胞有毒性作用的复杂次生代谢产物和物质。噬菌体尾样细菌素（尾菌素）是一种具有抗菌活性的化合物。然而，其在[具体内容缺失]中的功能尚未见报道。本文探究了WS-FJ9对植物病原真菌和卵菌的拮抗能力，筛选该菌株中潜在的尾菌素并验证其功能，以揭示其新的抗菌机制。我们发现WS-FJ9对植物病原真菌[具体真菌名称缺失]和[具体真菌名称缺失]以及病原卵菌[具体卵菌名称缺失]具有强烈的拮抗作用。从WS-FJ9菌株基因组中预测出噬菌体尾样蛋白Bm_67459。Bm_67459 cDNA编码111个氨基酸序列，相对分子质量约为11.69 kDa，理论等电点（pI）为5.49，是一种亲水性蛋白。Bm_67459没有跨膜螺旋区或信号肽，属于Phage_TAC_7超家族。qRT-PCR结果表明，在WS-FJ9与[具体对象缺失]接触期间，[具体基因缺失]基因表达显著上调。纯化的Bm_67459蛋白在10 μg·mL时能显著抑制[具体对象缺失]菌丝生长。综上所述，WS-FJ9菌株具有广谱抗植物病原菌活性，尾菌素Bm_67459是对抗植物病原菌[具体病原菌名称缺失]的重要效应因子，有助于在分子水平揭示该菌株的拮抗机制，并为植物病害的生物防治提供优良的菌株资源。
