Dărăbuș Rodica Georgiana, Imre Mirela, Dărăbuș Gheorghe, Ilie Marius Stelian, Olariu Alexander Tudor, Dărăbuș Diana Maria, Lăzureanu Voichița, Roșca Ovidiu, Olariu Tudor Rareș
Discipline of Parasitology, Department of Infectious Diseases, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania.
Center for Diagnosis and Study of Parasitic Diseases, Department of Infectious Disease, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania.
Microorganisms. 2025 Apr 17;13(4):931. doi: 10.3390/microorganisms13040931.
The study aimed to identify infection in hospitalized patients in Western Romania. Stool samples were collected from 175 patients, both male and female, aged between 2 months and 88 years, residing in urban and rural areas. The identification of was performed using a qualitative chromatographic rapid test, supplemented by the modified Ziehl-Neelsen method described by Henricksen and Pohlenz. spp. were identified through PCR analysis and Sanger sequencing. To assess potential risk factors for cryptosporidiosis, a questionnaire was administered to the study participants. Laboratory test results revealed a cryptosporidiosis occurrence of 7.42%, with a significantly higher occurrence observed in urban areas ( < 0.05). Two species were identified, and , the latter being reported for the first time in humans in Romania. Among the assessed risk factors, only the area of residence significantly influenced the occurrence of infection. The other evaluated risk factors-age, sex, concomitant microbial infections, contact with animals, use of public transport, international travel, frequenting children's playgrounds, and swimming pools-although potentially involved in infection, did not have a significant contribution. This study represents the first report of identified in humans in Romania. Our results indicate a high occurrence of human cryptosporidiosis in hospitalized patients, with a significantly higher rate observed in individuals residing in urban areas.
该研究旨在确定罗马尼亚西部住院患者中的感染情况。从175名患者中采集粪便样本，这些患者年龄在2个月至88岁之间，男女皆有，居住在城市和农村地区。使用定性色谱快速检测法进行鉴定，并辅以Henricksen和Pohlenz描述的改良齐尔-尼尔森方法。通过PCR分析和桑格测序鉴定隐孢子虫属物种。为评估隐孢子虫病的潜在危险因素，向研究参与者发放了问卷。实验室检测结果显示隐孢子虫病的发生率为7.42%，在城市地区观察到的发生率显著更高（P<0.05）。鉴定出两种物种，即微小隐孢子虫和罗马尼亚隐孢子虫，后者是首次在罗马尼亚人类中报道。在评估的危险因素中，只有居住地区对隐孢子虫感染的发生率有显著影响。其他评估的危险因素——年龄、性别、合并微生物感染、与动物接触、使用公共交通工具、国际旅行、经常去儿童游乐场和游泳池——虽然可能与隐孢子虫感染有关，但没有显著影响。这项研究是罗马尼亚首次关于人类隐孢子虫鉴定的报告。我们的结果表明住院患者中人类隐孢子虫病的发生率很高，居住在城市地区的个体中观察到的发生率显著更高。
