Martínez-Torres Martín, Sánchez-Rivera Uriel Ángel, Medrano Alfredo, Hernández-Gónzalez Enrique Othón, Dávila-Govantes Rodrigo, Castro-Camacho Yabín Josué, Cruz-Cano Norma Berenice
Laboratorio de Investigación de Saurios en Asistencia Reproductiva y Desarrollo (LISARD), Unidad de Morfología y Función, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios # 1, Los Reyes Iztacala, Tlanepantla 54090, Estado de Mexico, Mexico.
Laboratorio de Reproducción Animal, Unidad de Investigación Multidiciplinaria, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli 54714, Estado de Mexico, Mexico.
Vet Sci. 2025 Apr 13;12(4):363. doi: 10.3390/vetsci12040363.
While most lizard species are polygamous, dominant males typically have greater access to females and copulate more frequently than submissive ones. Several studies suggest that applying assisted reproduction techniques to this taxon could be an effective tool to enhance conservation programs. Therefore, the proper selection of males for assisted breeding programs is crucial for their overall success. In this study, we report for the first time data on semen and sperm quality from dominant males of the Mexican lizard using techniques commonly employed in assessing mammalian fertility. The semen and sperm characteristics were as follows: volume, 14.0 µL; sperm concentration, 125.7 × 10 cells/mL; wave motion, 2.7; total motility, 87.8%; sperm viability, 89.0%; normal morphology, 88.8%; plasma membrane integrity, 87.7%; low plasma membrane fluidity, 94.9%; capacitation status: uncapacitated (F), 90.5%; capacitated (B), 7.2%; sperm with acrosome reaction (AR), 2.3%; and, acrosome integrity, 88.8%. These results suggest that, according to mammalian fertility parameters, dominant males of are fertile. This series of tests provides a valuable tool for conducting a comprehensive analysis of the functional changes that may occur in sperm handling during assisted processes, and that may contribute-along with the proper selection of individuals-to reproductive success.
虽然大多数蜥蜴物种是一夫多妻制，但占主导地位的雄性通常比顺从的雄性有更多机会接触雌性，交配也更频繁。几项研究表明，对这个分类群应用辅助生殖技术可能是加强保护计划的有效工具。因此，为辅助繁殖计划正确选择雄性对其整体成功至关重要。在本研究中，我们首次报告了使用评估哺乳动物生育能力的常用技术对墨西哥蜥蜴占主导地位的雄性的精液和精子质量数据。精液和精子特征如下：体积，14.0微升；精子浓度，125.7×10个细胞/毫升；波动，2.7；总活力，87.8%；精子活力，89.0%；正常形态，88.8%；质膜完整性，87.7%；低质膜流动性，94.9%；获能状态：未获能（F），90.5%；获能（B），7.2%；发生顶体反应（AR）的精子，2.3%；顶体完整性，88.8%。这些结果表明，根据哺乳动物的生育参数，该蜥蜴的占主导地位的雄性具有生育能力。这一系列测试为全面分析辅助过程中精子处理可能发生的功能变化提供了有价值的工具，并且可能与个体的正确选择一起有助于生殖成功。
