Sousa Sêmilly Suélen da Silva, Cruz Ana Cecília Ribeiro, Aragão Carine Fortes, Cereja Glennda Juscely Galvão Pereira, Silva Sandro Patroca da, Sousa Raira Maria Morais de, Amorim Murilo Tavares, da Silva Eliana Vieira Pinto, Nunes Bruno Tardelli Diniz, Pinheiro Valéria Cristina Soares
Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Rede BIONORTE, Universidade Estadual do Maranhão-UEMA, São Luis 65055-310, Brazil.
Laboratório de Entomologia Médica-LABEM, Universidade Estadual do Maranhão-UEMA, Campus Caxias, Caxias 65604-380, Brazil.
Viruses. 2025 Mar 26;17(4):475. doi: 10.3390/v17040475.
Arboviruses transmitted by cause high number of cases and deaths annually. The aim was to investigate the presence of the presence of Dengue (DENV), Zika (ZIKV) and Chikungunya (CHIKV) viruses in endemic areas of Maranhão, northeastern Brazil. The study was carried out in Caxias, Codó, Peritoró, and São Mateus do Maranhão in 2019 (Caxias) and 2022. The blood samples were subjected to RNA extraction and then tested by RT-qPCR. Cell culture was used to attempt viral isolation and subsequent sequencing. In total, 171 samples were analyzed (32 from 2019, 18.7%) and 72 (42.1%) were found to have arboviruses: 68 (39.7%) from Caxias; 2 (1.1%) from Codó; 1 (0.6%) from Peritoró; and 1 (0.6%) from São Mateus. Overall, 85.3% ( = 58) of the positive samples were infected with DENV-1, 4 (four) (5.9%) with DENV-2 (Caxias), 1 (one) (1.5%) with DENV-3 (Caxias), and in 6 (six) (7.3%) samples CHIKV was detected, with one co-infection of DENV-1 and CHIKV (Caxias). The DENV-1 genotype V and the ECSA genotype of CHIKV were characterized in samples from Caxias. The detection of DENV-1, DENV-2, DENV-3, and more CHIKV in the interior of Maranhão alerts to the importance of virological studies in these areas.
由[传播媒介]传播的虫媒病毒每年导致大量病例和死亡。目的是调查巴西东北部马拉尼昂州流行地区登革热病毒（DENV）、寨卡病毒（ZIKV）和基孔肯雅病毒（CHIKV）的存在情况。该研究于2019年（卡希亚斯）和2022年在卡希亚斯、科多、佩里托罗和马拉尼昂州圣马特乌斯进行。对血样进行RNA提取，然后通过逆转录定量聚合酶链反应（RT-qPCR）进行检测。使用细胞培养尝试病毒分离及后续测序。总共分析了171份样本（2019年的32份，占18.7%），发现72份（42.1%）含有虫媒病毒：卡希亚斯的68份（39.7%）；科多的2份（1.1%）；佩里托罗的1份（0.6%）；圣马特乌斯的1份（0.6%）。总体而言，85.3%（n = 58）的阳性样本感染了DENV-1，4份（5.9%）感染了DENV-2（卡希亚斯），1份（1.5%）感染了DENV-3（卡希亚斯），6份（7.3%）样本检测到CHIKV，其中1份样本为DENV-1和CHIKV共感染（卡希亚斯）。在卡希亚斯的样本中鉴定出了DENV-1基因型V和CHIKV的东、中、南非洲基因型（ECSA）。在马拉尼昂州内陆地区检测到DENV-1、DENV-2、DENV-3以及更多的CHIKV，这提醒人们在这些地区进行病毒学研究的重要性。
