Aragão Carine Fortes, Cruz Ana Cecília Ribeiro, Nunes Neto Joaquim Pinto, Monteiro Hamilton Antonio de Oliveira, da Silva Eliana Vieira Pinto, da Silva Sandro Patroca, Andrade Aylane Tamara Dos Santos, Tadei Wanderli Pedro, Pinheiro Valéria Cristina Soares
Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ananindeua, PA, Brazil.
Acta Trop. 2018 Oct;186:1-4. doi: 10.1016/j.actatropica.2018.06.022. Epub 2018 Jun 19.
The simultaneous circulation of Dengue virus (DENV), Chikungunya virus (CHIKV) and Zika virus (ZIKV) arboviruses have placed Brazil among the main worldwide endemic areas. Brazilian Northeast region concentrates the highest incidence of infections caused by CHIKV and ZIKV. In Maranhão, the second biggest northeastern state, there are cases of human infections caused by these three arboviruses and presence of Aedes aegypti and Aedes albopictus vectors. In this context, this study aimed to investigate the circulation of CHIKV, DENV and ZIKV in Ae. aegypti and Ae. albopictus mosquitoes collected in urban areas of Barra do Corda, Caxias, Codó, São Luís and São Mateus do Maranhão municipalities in the state of Maranhão through Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) technique. 428 Ae. aegypti and 1 Ae. albopictus were collected, which formed 44 pools. Three of these showed positive results for CHIKV: AR832767 (five Ae. aegypti female collected in Caxias), AR832784 and AR832785 (both composed of 20 Ae. aegypti female collected in São Mateus do Maranhão). This study consolidates information about CHIKV circulation in state of Maranhão, as well as the role of Ae. aegypti in the transmission of CHIKV in urban area.
登革热病毒（DENV）、基孔肯雅病毒（CHIKV）和寨卡病毒（ZIKV）这三种虫媒病毒的同时传播，使巴西成为全球主要的流行地区之一。巴西东北部地区是基孔肯雅病毒和寨卡病毒感染发病率最高的地区。在东北部第二大州马拉尼昂州，存在这三种虫媒病毒导致的人类感染病例，并且有埃及伊蚊和白纹伊蚊这两种病媒。在此背景下，本研究旨在通过定量逆转录聚合酶链反应（RT-qPCR）技术，调查在马拉尼昂州巴拉多科尔达、卡西亚斯、科多、圣路易斯和圣马特乌斯-杜马拉尼昂等市城区采集的埃及伊蚊和白纹伊蚊中基孔肯雅病毒、登革热病毒和寨卡病毒的传播情况。共采集了428只埃及伊蚊和1只白纹伊蚊，形成了44个样本池。其中3个样本池的基孔肯雅病毒检测结果呈阳性：AR832767（在卡西亚斯采集的5只埃及伊蚊雌蚊）、AR832784和AR832785（均由在圣马特乌斯-杜马拉尼昂采集的20只埃及伊蚊雌蚊组成）。本研究巩固了关于基孔肯雅病毒在马拉尼昂州传播的信息，以及埃及伊蚊在城区基孔肯雅病毒传播中的作用。
