Groch Kátia Regina, Miyagi Sueli Akemi Taniwaki, Díaz-Delgado Josué, Santos-Neto Elitieri B, Lailson-Brito José, Brandão Paulo Eduardo, Catão-Dias José Luiz
Department of Pathology, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo 05508-207, Brazil.
Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo 05508-207, Brazil.
Viruses. 2025 Apr 18;17(4):582. doi: 10.3390/v17040582.
Cetacean morbillivirus (CeMV) is a major threat to cetaceans worldwide, causing individual deaths and outbreaks of mass mortality. Based on partial sequences of the viral phosphoprotein, CeMV is subclassified into seven strains and two distinct lineages. To date, only CeMV-1 strains, including the dolphin morbillivirus (DMV), have been completely sequenced. The CeMV-2 lineage was first reported in Guiana dolphins () in Brazil and was associated with an unusual mortality event in 2017-2018. Here we provide the nearly complete Guiana dolphin morbillivirus (GDMV) genome sequence, representing the first within the CeMV-2 lineage. GDMV was isolated using Vero.DogSLAMtag cells, the viral RNA was extracted, and deep sequencing analysis was performed. Gaps in the viral genome were completed by Sanger sequencing. The final genome length was 15,607 nucleotides covering 99.3% of the DMV reference genome, including full sequences of the six structural proteins encoded by morbillivirus. The sequence similarity was 74-77.9% to other CeMV strains, with highest identity to the DMV. The complete L protein amino acid sequence comparison-based taxonomy indicates that GDMV is a distinct morbillivirus species; however, as GDMV and CeMV-1 strains infect a similar host spectrum, our findings support that GDMV represents a new CeMV-2 lineage.
鲸目动物麻疹病毒（CeMV）是全球鲸目动物面临的主要威胁，可导致个体死亡和大规模死亡疫情。根据病毒磷蛋白的部分序列，CeMV被细分为七个毒株和两个不同的谱系。迄今为止，只有包括海豚麻疹病毒（DMV）在内的CeMV-1毒株已被完全测序。CeMV-2谱系首次在巴西的圭亚那海豚中被报道，并与2017 - 2018年的一次异常死亡事件有关。在此，我们提供了近乎完整的圭亚那海豚麻疹病毒（GDMV）基因组序列，这是CeMV-2谱系中的首个序列。使用Vero.DogSLAMtag细胞分离出GDMV，提取病毒RNA，并进行深度测序分析。病毒基因组中的缺口通过桑格测序完成。最终基因组长度为15607个核苷酸，覆盖了DMV参考基因组的99.3%，包括麻疹病毒编码的六种结构蛋白的完整序列。与其他CeMV毒株的序列相似性为74 - 77.9%，与DMV的同一性最高。基于完整L蛋白氨基酸序列比较的分类学表明，GDMV是一种独特的麻疹病毒物种；然而，由于GDMV和CeMV-1毒株感染相似的宿主谱，我们的研究结果支持GDMV代表一个新的CeMV-2谱系。
