Corrêa-Moreira Danielly, da Costa Gisela Lara, Pinto Tatiane Nobre, Alves Julia Araujo, Martins Laura Brandão, Zahner Viviane, Chagas Thiago Pavoni Gomes, Amorim Milena Conceição, de Sá Salomão André Luís, Saggioro Enrico Mendes, Oliveira Manoel Marques Evangelista
Laboratory of Taxonomy, Biochemistry, and Bioprospecting of Fungi, Oswaldo Cruz Institute - Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, 21040 - 360, Brazil.
Laboratory of Assessment and Promotion of Environmental Health, Oswaldo Cruz Institute - Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, 21040 - 360, Brazil.
Environ Monit Assess. 2025 Apr 26;197(5):596. doi: 10.1007/s10661-025-14022-5.
This study aimed to detect and identify emerging pathogenic yeasts in surface waters from two Lagoon Systems in Rio de Janeiro, Brazil, by polyphasic taxonomy. The monitoring of potentially pathogenic fungi in aquatic environments, especially in regions impacted by human actions, is highlighted in this study as one of the axes of the One Health approach. Water samples were collected, and after isolation, fungi were identified by polyphasic taxonomy, including MALDI-TOF-MS, which is also used for bacteria identification. Our results describe the physicochemical parameters of the surface water of these lagoons and reveal a significant diversity of yeast species, some of which are known to exhibit pathogenic potential and resistance to common antifungal treatments, as well as bacteria with resistance profiles to several currently used antibiotics. We conclude that Jacarepaguá Lagoon is the most impacted compared to Piratininga Lagoon; however, we highlight the urgent need for targeted interventions to mitigate pollution in both Lagoons, reinforcing the value of long-term surveillance in both ecosystems. We believe that monitoring emerging species is as critical as analyzing traditional water quality parameters. Early detection of resistant or invasive pathogens in aquatic ecosystems can prevent disease outbreaks, protect biodiversity, and mitigate public health risks, preventing loss of life, and optimizing public healthcare system expenditures.
本研究旨在通过多相分类法检测和鉴定巴西里约热内卢两个泻湖系统地表水中新出现的致病性酵母。本研究强调，对水生环境，特别是受人类活动影响地区的潜在致病真菌进行监测，是“同一健康”方法的核心要点之一。采集水样后，通过多相分类法对分离出的真菌进行鉴定，包括用于细菌鉴定的基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）。我们的研究结果描述了这些泻湖地表水的理化参数，并揭示了酵母种类的显著多样性，其中一些已知具有致病潜力且对常见抗真菌治疗具有抗性，同时还发现了对几种目前使用的抗生素具有抗性的细菌。我们得出结论，与皮拉蒂宁加泻湖相比，雅卡雷帕瓜泻湖受到的影响最大；然而，我们强调迫切需要采取有针对性的干预措施来减轻两个泻湖的污染，加强对这两个生态系统进行长期监测的重要性。我们认为，监测新出现的物种与分析传统水质参数同样重要。在水生生态系统中早期发现耐药或入侵病原体可以预防疾病爆发、保护生物多样性并降低公共卫生风险，避免生命损失，优化公共医疗系统支出。
