Yang Suyu, Gao Bowen, Yang Qingrui, Huo Yanghui, Li Kailin, Shu Liangyin, Fan Lingxuan, Liu Yiliang, Li Huanting, Cai Wei
School of Pharmacy, Baotou Medical College, Baotou 014040, China.
School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China.
Molecules. 2025 Mar 24;30(7):1433. doi: 10.3390/molecules30071433.
A Hua (PCH) is a common medicinal and edible plant whose rhizomes are widely used for the treatment and prevention of various diseases. Previous studies have revealed a variety of chemical components such as polysaccharides, saponins, and flavonoids, which possess a variety of biological activities such as antimicrobial, lipid-regulating, anti-aging, hypoglycemic, and anti-inflammatory. However, to date, the structure and activity of its oligosaccharide components are still unclear. In this study, we developed a method combining ultra-high-performance liquid chromatography with Q-Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap MS) and monosaccharide analysis for the identification of oligosaccharides in PCH. Finally, a total of 44 oligosaccharides, including 27 fructo-oligosaccharides (FOS), 10 arabino-oligosaccharides (AOS), and 7 others, were identified based on the precise relative molecular mass and fragment ion information provided by high-resolution mass spectrometry, in combination with standard comparison, monosaccharide composition analysis, and the relevant literature reports. All of those oligosaccharides were reported for the first time. These findings laid the foundation for the subsequent study of its medicinal substances and provided a theoretical basis for the more comprehensive development and utilization of PCH as a medicinal and edible product.
A华（PCH）是一种常见的药食两用植物，其根茎被广泛用于治疗和预防各种疾病。先前的研究已经揭示了多种化学成分，如多糖、皂苷和黄酮类化合物，它们具有多种生物活性，如抗菌、调节血脂、抗衰老、降血糖和抗炎。然而，迄今为止，其寡糖成分的结构和活性仍不清楚。在本研究中，我们开发了一种将超高效液相色谱与Q-Exactive Orbitrap质谱（UHPLC-Q-Exactive Orbitrap MS）相结合的方法以及单糖分析方法来鉴定PCH中的寡糖。最后，根据高分辨率质谱提供的精确相对分子质量和碎片离子信息，结合标准比较、单糖组成分析和相关文献报道，共鉴定出44种寡糖，其中包括27种低聚果糖（FOS）、10种阿拉伯低聚糖（AOS）和7种其他寡糖。所有这些寡糖均为首次报道。这些发现为其药用物质的后续研究奠定了基础，并为PCH作为药食两用产品的更全面开发利用提供了理论依据。
