Huang Chen, Song Xiaojin, Li Jingyi, Cui Qiu, Gu Pengfei, Feng Yingang
School of Biological Science and Technology, University of Jinan, Jinan 250022, China.
CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
Molecules. 2025 Apr 7;30(7):1646. doi: 10.3390/molecules30071646.
Squalene is an important bioactive substance widely used in the food, pharmaceutical, and cosmetic industries. Microbial production of squalene has gained prominence in recent years due to its sustainability, safety, and environmental friendliness. In this study, a mutant strain, sp. P4-22, with enhanced squalene-producing ability, was obtained through atmospheric and room temperature plasma mutagenesis of the previously screened squalene-producing yeast sp. SD301. The P4-22 strain demonstrated the ability to produce squalene using various carbon and nitrogen sources. We optimized the culture conditions by employing cost-effective corn steep liquor as the nitrogen source, and the optimal pH and sea salt concentration of the medium were determined to be 5.5 and 5 g/L, respectively. Under optimal cultivation conditions, the biomass and squalene production reached 64.42 g/L and 2.06 g/L, respectively, in a 5 L fed-batch fermentation. This study highlights the potential of sp. P4-22 as a promising strain for commercial-scale production of squalene.
角鲨烯是一种重要的生物活性物质，广泛应用于食品、制药和化妆品行业。近年来，由于其可持续性、安全性和环境友好性，微生物生产角鲨烯受到了广泛关注。在本研究中，通过对先前筛选出的产角鲨烯酵母菌株SD301进行常压室温等离子体诱变，获得了一株角鲨烯生产能力增强的突变菌株P4-22。P4-22菌株能够利用多种碳源和氮源生产角鲨烯。我们采用经济高效的玉米浆作为氮源优化了培养条件，并确定培养基的最佳pH值和海盐浓度分别为5.5和5 g/L。在最佳培养条件下，5 L补料分批发酵中生物量和角鲨烯产量分别达到64.42 g/L和2.06 g/L。本研究突出了P4-22菌株作为角鲨烯商业规模生产有前景菌株的潜力。
