Lammintausta K, Kalimo K, Jansén C T
Contact Dermatitis. 1985 May;12(5):258-62. doi: 10.1111/j.1600-0536.1985.tb01132.x.
3 different sensitization protocols were compared for inducing delayed-type nickel contact hypersensitivity in guinea pigs. Open epicutaneous sensitization (OE) induced nickel allergy in 11/22 (50%) guinea pigs. When intradermal injections of Freund's complete adjuvant into the nickel-painted skin was added to the same protocol, 4/13 (31%) became sensitized. The guinea pig maximization protocol induced nickel allergy in only 7/31 (23%) of the animals. Compared with the 2 other methods, animals sensitized with open epicutaneous applications reacted more rapidly (maximum at 6 h) and strongly (2+ reaction in 12/22 of animals) in previous patch test sites upon systemic (i.p.) nickel challenge. Open epicutaneous sensitization of guinea pigs should be a useful model for studying cellular and immunological mechanisms in nickel contact sensitivity.
比较了3种不同的致敏方案在豚鼠中诱导迟发型镍接触超敏反应的效果。开放性皮肤致敏（OE）使22只豚鼠中的11只（50%）产生了镍过敏。当在相同方案中加入在涂有镍的皮肤上皮内注射弗氏完全佐剂时，13只中有4只（31%）致敏。豚鼠最大化方案仅使31只动物中的7只（23%）产生了镍过敏。与其他两种方法相比，经开放性皮肤应用致敏的动物在全身性（腹腔内）镍激发后，先前斑贴试验部位的反应更快（6小时达到峰值）且更强（22只动物中有12只出现2+反应）。豚鼠开放性皮肤致敏应是研究镍接触敏感性细胞和免疫机制的有用模型。
