Lammintausta K, Pitkänen O P, Kalimo K, Jansen C T
Contact Dermatitis. 1985 Sep;13(3):148-52. doi: 10.1111/j.1600-0536.1985.tb02527.x.
The possible modulating effect of previous nickel sensitization on subsequent cobalt sensitization, and vice versa, was studied in a guinea pig model, using an open epicutanous induction protocol. Challenge tests were made by both topical and systemic routes. Controls included animals sensitized to only one of the metals. Animals sensitized with both metals seemed to react more readily in patch testing to either allergen, as compared to mono-sensitized animals. Systemic challenge with cobalt revealed a significantly (p less than 0.05) higher reaction rate (70%) in animals originally sensitized to cobalt and subsequently to nickel, as compared to the reaction rate (20%) in cobalt mono-sensitized animals. The reverse order of double-sensitization (nickel first, cobalt subsequently) led to an intermediate cobalt sensitization rate (50%). These experimental data corroborate clinical findings which have indicated a mutual enhancing effect of nickel and cobalt contact sensitization in man, and provide an animal model for studying the underlying immunological processes.
在豚鼠模型中，采用开放性表皮诱导方案，研究先前镍致敏对随后钴致敏的可能调节作用，反之亦然。通过局部和全身途径进行激发试验。对照组包括仅对其中一种金属致敏的动物。与单一致敏动物相比，对两种金属均致敏的动物在斑贴试验中似乎对任何一种变应原的反应都更迅速。对钴进行全身激发试验显示，最初对钴致敏随后又对镍致敏的动物的反应率（70%）显著高于（p<0.05）钴单一致敏动物的反应率（20%）。双重致敏的相反顺序（先镍后钴）导致钴致敏率处于中间水平（50%）。这些实验数据证实了临床研究结果，即镍和钴接触致敏在人类中具有相互增强作用，并为研究潜在的免疫过程提供了一个动物模型。
