Xu Jiao, Huang Xuan, Wang Xiuwen, Li Shengnan, Ou Xiaohong, Yuan Qingsong, Wang Yanhong, He Hua, Jiang Weike, Zhou Tao
Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, China.
Guizhou Key Laboratory for Germplasm Innovation and Resource-Efficient Utilization of Dao-di Herbs, Guiyang, 550025, China.
BMC Microbiol. 2025 Apr 26;25(1):247. doi: 10.1186/s12866-025-03907-7.
Armillaria gallica establish a unique symbiotic relationship with Gastrodia elata, which is a valuable Chinese herbal plant that relies on this symbiosis. High temperature (HT) seriously affected the growth of both A. gallica and G. elata. However, the molecular mechanisms and gene networks involved in the response of A. gallica to HT are not well understood.
In this study, transcriptomic analyses at multiple time point were conducted using rhizomorph from two A. gallica strains, GZ1 (tolerance to HT) and SX8 (sensitive to HT) under HT and normal temperature (NT) conditions. We identified 2,056 differentially expressed genes, primarily associated with carbohydrate metabolism and protein processing in endoplasmic reticulum (ER). Heat shock proteins (HSP20 and HSP90) were up-regulated in both GZ1 and SX8 under HT, indicating a conserved HT induced response in A. gallica. Additionally, genes related to glycolysis were up-regulated in GZ1 following HT treatment, while genes involved in the conversion of sugar to amino acids were down-regulated in GZ1 under HT treatment. GZ1 also showed an increased accumulation of soluble sugar and polysaccharide under HT, which were significantly correlated with pyruvate kinase and aldo/keto reductase genes. Furthermore, a sucrose concentration of 30 g/L enhanced HT resistance in A. gallica.
These results reveal that sugar signaling, particularly in carbohydrate metabolism, plays a critical role in the rhizomorph response to HT stress. Both conserved and species-specific transcriptome changes may contribute to the dynamic regulation of rhizomorph development during adaptation to HT stress in A. gallica.
Not applicable.
蜜环菌与天麻建立了独特的共生关系，天麻是一种珍贵的中药材，依赖这种共生关系生长。高温严重影响蜜环菌和天麻的生长。然而，蜜环菌对高温响应所涉及的分子机制和基因网络尚不清楚。
在本研究中，使用来自两株蜜环菌菌株（GZ1，耐高温；SX8，对高温敏感）的菌索，在高温和常温条件下进行了多个时间点的转录组分析。我们鉴定出2056个差异表达基因，主要与碳水化合物代谢和内质网中的蛋白质加工相关。热休克蛋白（HSP20和HSP90）在高温下的GZ1和SX8中均上调，表明蜜环菌中存在保守的高温诱导反应。此外，高温处理后，GZ1中与糖酵解相关的基因上调，而参与糖向氨基酸转化的基因在高温处理下的GZ1中下调。GZ1在高温下还表现出可溶性糖和多糖积累增加，这与丙酮酸激酶和醛糖/酮糖还原酶基因显著相关。此外，30 g/L的蔗糖浓度增强了蜜环菌的耐高温性。
这些结果表明，糖信号，特别是在碳水化合物代谢中，在菌索对高温胁迫的响应中起关键作用。保守的和物种特异性的转录组变化可能有助于蜜环菌在适应高温胁迫过程中对菌索发育的动态调节。
不适用。
