Cheng Rui, Liu Tian-Rui, Li Yang-Hua, Zhou Jun-Hui, Zhao Yu-Yang, Yuan Yuan
School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China.
State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences Nanchang 330115, China.
Zhongguo Zhong Yao Za Zhi. 2023 Jan;48(2):374-381. doi: 10.19540/j.cnki.cjcmm.20221014.101.
This study aims to screen a strain from Armillaria for the cultivation of Gastrodia elata. Specifically, Armillaria strains were isolated from different producing areas of G. elata and identified. Based on the growth characteristics of the strains and the experiment on the cultivation of G. elata, an optimal A. gallica strain was screened out. The specific process is as follows. The fungus-gro-wing materials of G. elata were collected from four producing areas and the Armillaria strains were isolated(G,Y,S,H). The strains were then identified based on morphological observation and phylogeny analysis and the commonly used strains were determined. The sucrase genotypes of the strains were identified according to our previous research findings, and the growth characteristics of the strains, such as growth rate, diameter, dry weight, and polysaccharide content of the rhizomorphs, were measured. According to the biological characteristics and sucrase genotypes, two strains were selected for the cultivation of G. elata. The tuber yield and the content of gastrodin and p-hydroxybenzyl alcohol in the tuber of G. elata were measured to select the optimal strain. The results showed that the four strains were all A. gallica. The rhizomorphs of strains G and H of the same sucrase genotype had larger/higher length, growth rate, diameter, branch number, dry weight, and polysaccharide content than those of strains S and Y of the same sucrase genotype. The tuber yield and the total content of gastrodin and p-hydroxybenzyl alcohol in tuber of G. elata cultivated with strain H were 6.528 kg·m~(-2) and 0.566%, respectively, which were 4.58 and 1.30 folds those of G. elata cultivated with strain S. Strains H and S were screened out from four strains of A. gallica based on the growth characteristics and sucrase genotype. According to the tuber yield and content of total gastrodin and p-hydroxybenzyl alcohol in the tuber of G. elata, strain H was identified as the optimal one. The findings in this study are expected to lay a basis for cultivating G. elata with high yield and quality of tubers.
本研究旨在从蜜环菌中筛选出用于栽培天麻的菌株。具体而言，从不同天麻产区分离蜜环菌菌株并进行鉴定。基于菌株的生长特性以及天麻栽培试验，筛选出最优的高卢蜜环菌菌株。具体过程如下。从四个产区收集天麻的菌材并分离蜜环菌菌株（G、Y、S、H）。然后基于形态学观察和系统发育分析对菌株进行鉴定，并确定常用菌株。根据我们之前的研究结果鉴定菌株的蔗糖酶基因型，并测定菌株的生长特性，如菌索的生长速率、直径、干重和多糖含量。根据生物学特性和蔗糖酶基因型，选择两个菌株用于栽培天麻。测定天麻块茎产量以及天麻素和对羟基苯甲醇在天麻块茎中的含量，以筛选出最优菌株。结果表明，这四个菌株均为高卢蜜环菌。相同蔗糖酶基因型的G菌株和H菌株的菌索长度、生长速率、直径、分支数、干重和多糖含量比相同蔗糖酶基因型的S菌株和Y菌株更大/更高。用H菌株栽培的天麻块茎产量以及天麻素和对羟基苯甲醇的总含量分别为6.528 kg·m⁻²和0.566%，分别是用S菌株栽培的天麻的4.58倍和1.30倍。基于生长特性和蔗糖酶基因型从四个高卢蜜环菌菌株中筛选出H菌株和S菌株。根据天麻块茎产量以及天麻素和对羟基苯甲醇在天麻块茎中的总含量，确定H菌株为最优菌株。本研究结果有望为高产优质天麻的栽培奠定基础。
