Yan Zhiyu, Zhao Mengran, Wu Xiangli, Zhang Jinxia
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
Key Laboratory of Microbial Resources, Ministry of Agriculture and Rural Affairs, Beijing, China.
Front Microbiol. 2020 Jan 21;10:3148. doi: 10.3389/fmicb.2019.03148. eCollection 2019.
Heat stress seriously threatens the growth of . Various studies have been performed to study the resistance of to heat stress. Here, the metabolome was evaluated to determine the response of mycelia to heat stress at different times (6, 12, 24, 48 h). More than 70 differential metabolites were detected and enriched in their metabolic pathways. Dynamic metabolites changes in enrichment pathways under heat stress showed that heat stress enhanced the degradation of unsaturated fatty acids and nucleotides, increased the content of amino acids and vitamins, and accelerated glycolysis and the tricarboxylic acid cycle in . The time course changes of metabolites under continuous heat stress demonstrated that amino acids continuously changed with heat stress, nucleotides clearly changed with heat stress at 12 and 48 h, and lipids exhibited an increasing trend with prolonged heat stress, while few types saccharides and vitamins changed under heat stress. Additionally, heat-treated produced salicylic acid and other stress-resistant substances that were reported in plants. This study first reported the metabolites changes in mycelia during 48 h of heat stress. The metabolic pathways and substances that changed with heat stress in this research will aid future studies on the resistance of and other edible fungi to heat stress.
热胁迫严重威胁着……的生长。已开展了多项研究来探究……对热胁迫的抗性。在此，对代谢组进行了评估，以确定……菌丝体在不同时间点（6、12、24、48小时）对热胁迫的响应。检测到70多种差异代谢物，并在其代谢途径中得到富集。热胁迫下富集途径中代谢物的动态变化表明，热胁迫增强了不饱和脂肪酸和核苷酸的降解，增加了氨基酸和维生素的含量，并加速了……中的糖酵解和三羧酸循环。连续热胁迫下……代谢物的时间进程变化表明，氨基酸随热胁迫持续变化，核苷酸在12小时和48小时时随热胁迫明显变化，脂质随热胁迫时间延长呈增加趋势，而少数糖类和维生素在热胁迫下变化不大。此外，经热处理的……产生了植物中报道的水杨酸和其他抗逆物质。本研究首次报道了……菌丝体在48小时热胁迫期间的代谢物变化。本研究中随热胁迫而变化的代谢途径和物质将有助于未来对……及其他食用菌抗热胁迫的研究。
